TAMRA phosphoramidite, 5-isomer

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TAMRA phosphoramidite, 5-isomer

标签:Oligonucleotide synthesis,Phosphoramidite,TAMRA (TMR, tetramethylrhodamine)
货号 规格 价格 货期
2497-100mg 100 mg 10 days
2497-250mg 250 mg 350.00$ in stock
2497-1g 1 g 890.00$ in stock
2497-5g 5 g please inquire 21 days


This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.

TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).

TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.

The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.

该亚磷酰胺用于合成 5′ 标记的 TAMRA 寡核苷酸。

TAMRA(羧基四甲基罗丹明)是罗丹明家族的一种呫吨染料,在橙色光谱范围内发射(最大波长为 563 nm)。这种荧光团传统上用作 FRET 受体(和猝灭剂),与荧光素 (FAM) 成对使用,因为它们的光谱有显着重叠。因此,这种亚磷酰胺便于合成双标记探针 TaqMan,该探针在序列中间或 3′ 端含有 5′-末端 TAMRA 和 FAM(分别使用荧光素 dT 亚磷酰胺和 FAM CPG)。

TAMRA 5′ 标记的寡核苷酸通常用于定量 PCR 和片段分析(例如,用于微卫星标记分析),因为可用的设备经常具有 TAMRA 检测通道。

TAMRA 染料在存在铵和空间位阻伯胺时不稳定,因此强烈建议遵循特定条件进行标记的寡核苷酸脱保护。


Coupling: 7.5 min.

Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3 (v/v/v) («TAMRA cocktail») for 6 hours at 60 °C, then cool down to room temperature.

Due to complete and irreversible degradation of the TAMRA dye, do NOT use aqueous ammonium and AMA for deprotecting a modified oligonucleotide from the solid-phase support.

耦合:7.5 分钟。

脱保护:三丁胺:甲醇:水 1 : 1 : 3 (v/v/v)(《TAMRA 鸡尾酒》)在 60°C 下 6 小时,然后冷却至室温。

由于 TAMRA 染料会完全且不可逆地降解,因此请勿使用铵水溶液和 AMA 将修饰的寡核苷酸从固相载体上脱去保护。

TAMRA phosphoramidite, 5-isomer TAMRA phosphoramidite, 5-isomer TAMRA phosphoramidite, 5-isomer TAMRA phosphoramidite, 5-isomer

Absorption and emission spectra of 5-TAMRA

TAMRA phosphoramidite, 5-isomer

TAMRA phosphoramidite, 5-isomer相关产品

DusQ1 CPG 500

Controlled pore glass (CPG) 500 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.

可控孔玻璃 (CPG) 500 固体支持物,用于合成具有 3′-末端 DusQ1 淬灭剂的寡核苷酸。 与标准解块条件兼容。

BDP R6G azide

BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.

BDP R6G 是一种高性能硼二吡咯亚甲基荧光团,经过调整以匹配 R6G 染料的激发和发射通道。

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

General properties

Appearance: purple solid
Molecular weight: 727.83
Molecular formula: C40H50N5O6P
Solubility: good in acetonitrile, dichloromethane
Quality control: NMR 1H, HPLC-MS (95%), coupling test
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
CF260: 0.32
CF280: 0.19

Oligo synthesis details

Diluent: acetonitrile