JOE phosphoramidite, 6-isomer

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JOE phosphoramidite, 6-isomer

标签:Oligonucleotide synthesis, Phosphoramidite, JOE
货号 规格 价格 货期
12160 100 mg in stock
D2160 250 mg 170.00$ 10 days
F2160 1 g 590.00$ in stock
G2160 5 g 2290.00$ in stock
H2160 10 g 3390.00$ in stock
K2160 20 g please inquire 10 days

JOE phosphoramidite for oligonucleotide synthesis, pure 6-isomer (6-JOE).

Fluorescent dye JOE is a fluorescein derivative containing two chlorine atoms and two methoxy groups. Its absorption and emission maxima are at 503 nm and 525 nm, respectively. By its spectral characteristics JOE is found in between FAM and TAMRA/ROX; therefore, this fluorophor is commonly used for multiplex detection, including that during DNA sequencing.

Our catalog also contains JOE phosphoramidite, 5-isomer. We compared qPCR probes containing different JOE isomers (5-JOE and 6-JOE) and did not find any significant differences between them.

用于寡核苷酸合成的 JOE 亚磷酰胺,纯 6-异构体 (6-JOE)。

荧光染料 JOE 是一种含有两个氯原子和两个甲氧基的荧光素衍生物。 其吸收和发射最大值分别为 503 nm 和 525 nm。 根据其光谱特性,JOE 介于 FAM 和 TAMRA/ROX 之间; 因此,这种荧光团通常用于多重检测,包括 DNA 测序期间的检测。

我们的目录还包含 JOE 亚磷酰胺,5 异构体。 我们比较了含有不同 JOE 异构体(5-JOE 和 6-JOE)的 qPCR 探针,并没有发现它们之间有任何显着差异。


Coupling: 6 minutes

Deprotection: standard conditions using ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 h at 55 °С removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% of non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (e.g. 10 min at 65 °C).

耦合:6 分钟

脱保护:使用氢氧化铵的标准条件; 脱保护时间取决于寡核苷酸组成和核碱基保护基团(在 55°С 脱保护 17 小时会去除标准核碱基上的所有保护基团)。 AMA(30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v 的溶液)可用于形成约 5% 的非荧光副产物。 为避免形成副产物,用氢氧化铵开始脱保护(室温下 30 分钟),然后加入等体积的 40% 甲胺水溶液,并根据需要使用 AMA 继续脱保护(例如,在 65°C 下 10 分钟)。

JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer JOE phosphoramidite, 6-isomer

Absorption and emission spectra of JOE

JOE phosphoramidite, 6-isomer

JOE phosphoramidite, 6-isomer相关产品

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.

Cy5.5 NHS 是远红/近红外胺活性染料。

BDP R6G azide

BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.

BDP R6G 是一种高性能硼二吡咯亚甲基荧光团,经过调整以匹配 R6G 染料的激发和发射通道。

General properties

Appearance: white solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile