JOE phosphoramidite, 5-isomer

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JOE phosphoramidite, 5-isomer

标签:Oligonucleotide synthesis, Phosphoramidite ,JOE
货号 规格 价格 货期
C1160 100 mg 5 days
D1160 250 mg 170.00$ 5 days
F1160 1 g 590.00$ in stock
G1160 5 g 2290.00$ in stock
H1160 10 g 3390.00$ in stock

JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.

The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.

JOE 是一种呫吨染料,具有两个氯和两个甲氧基取代基的荧光素衍生物。 这种荧光团是寡核苷酸的有用标记。 其吸收和发射光谱位于 FAM 和 TAMRA 之间。

可以使用这种亚磷酰胺将荧光团引入寡核苷酸中。 它可以承受标准的铵解封条件。 本产品含有5-JOE染料的纯异构体。

JOE phosphoramidite, 5-isomer JOE phosphoramidite, 5-isomer JOE phosphoramidite, 5-isomer

Absorption and emission spectra of JOE dye

JOE phosphoramidite, 5-isomer

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Sulfo-Cyanine5.5 azide

Sulfo-Cyanine5.5 azide is a derivative of water soluble far red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper free Click chemistry. The reagent possesses high aqueous solubility and hydrophilicity, and recommended for the labeling of biomolecules in aqueous environment.

Sulfo-Cy5.5 azide 是水溶性远红荧光花青染料的衍生物。 叠氮基可以通过铜催化或无铜点击化学进行缀合。 该试剂具有高水溶性和亲水性,推荐用于水环境中生物分子的标记。

Sulfo-Cyanine5.5 amine

Sulfo-Cyanine5.5 is a far red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. The dye displays good brightness in far red region due to outstanding molar extinction coefficient. This is amine derivative which is reactive towards electrophiles. It can also be used for enzymatic transamination labeling.

Sulfo-Cy5.5 是一种远红/近红外荧光团,具有高亲水性和水溶性。 由于出色的摩尔消光系数,该染料在远红色区域显示出良好的亮度。 这是对亲电试剂有反应性的胺衍生物。 它也可用于酶促转氨标记。

General properties

Appearance: colorless solid
Molecular weight: 972.88
Molecular formula: C48H60N3Cl2O12P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases