Cyanine3 phosphoramidite 5′

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Cyanine3 phosphoramidite 5′

标签:Cy3, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2771-100mg 100 mg in stock
2771-250mg 250 mg 290.00$ 10 days
2771-1g 1 g 950.00$ in stock
2771-10g 10 g 6750.00$ 21 days

Cyanine3 is a fluorophore that is widely used in molecular biology experiments such as oligonucleotide labeling followed by oligonucleotide detection. By its spectral characteristics, Cyanine3 is an analog of Cy™3 with a fluorescence maximum at 570 nm in the yellow spectrum range.

Cyanine3 phosphoramidite 5’ is used in oligonucleotide synthesis for the production of 5’-cyanine3-labeled oligonucleotides. The reagent is compatible with various oligonucleotide synthesizers.

This phosphoramidite can be used for the synthesis of fluorescence-labeled primers and hybridization probes such as TaqMan and Molecular Beacon. Such labeled probes can be detected in multiplex real-time PCR in the TAMRA channel.

Condensation: 3 min. Use 0.02 M iodine solution at the oxidation step to avoid degradation of the cyanine dye.

Deprotection: At room temperature with 30% aqueous ammonium solution. It is recommended to use nucleic bases with labile protective groups for deprotection for not more than 2 h at less than 55°C. AMA (30% aqueous ammonium solution/40% aqueous methylamine 1:1 (v/v)) can be used for 10 min at 65°C in the presence of acetyldeoxycytidine. If deoxyguanidine with a dimethylformamidine protective group is used during synthesis, deprotect with 30% aqueous ammonium solution for 2 h at 65°C. If deoxyguanidine with an isobutyryl protective group is used during synthesis, deprotect for 24-36 h at room temperature.

Cy3 是一种荧光团,广泛用于分子生物学实验,例如寡核苷酸标记和寡核苷酸检测。根据其光谱特性,Cyanine3 是 Cy™3 的类似物,在黄色光谱范围内的荧光最大值为 570 nm。

花青 3 亚磷酰胺 5′ 用于寡核苷酸合成,以生产 5′-花青 3 标记的寡核苷酸。该试剂与各种寡核苷酸合成仪兼容。

这种亚磷酰胺可用于合成荧光标记的引物和杂交探针,例如 TaqMan 和 Molecular Beacon。可以在 TAMRA 通道中的多重实时 PCR 中检测到此类标记的探针。

冷凝:3 分钟。在氧化步骤中使用 0.02 碘溶液以避免花青染料降解。

脱保护:在室温下用 30% 的铵水溶液。建议使用带有不稳定保护基团的核酸碱基在低于 55°C 的条件下脱保护不超过 2 小时。 AMA(30% 铵水溶液/40% 甲胺水溶液 1:1 (v/v))可在乙酰脱氧胞苷存在下在 65°C 下使用 10 分钟。如果在合成过程中使用带有二甲基甲脒保护基团的脱氧胍,则在 65°C 下用 30% 铵水溶液脱保护 2 小时。如果在合成过程中使用带有异丁酰基保护基团的脱氧胍,则在室温下脱保护 24-36 小时。

Cyanine3 phosphoramidite 5’相关产品

Cyanine5.5 phosphoramidite, 5′-terminal

Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5′-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.

Amidite 用于合成带有 5′-末端染料 Cyanine5.5 的寡核苷酸和 qPCR 探针。 用于多重 qPCR 的有用染料。

FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

用于合成修饰寡核苷酸的单一异构体 6-FAM 亚磷酰胺。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cyanine5 的亚磷酰胺衍生物,用于寡核苷酸合成。

General properties

Appearance: red powder
Molecular weight: 841.81
Molecular formula: C45H65N5BF4O3P
Solubility: good in acetonitrile, dichloromethane
Quality control: NMR 1H, 31P, HPLC-MS (80%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

建议在 +4°C 或超温和保护基团下放置 48 小时; 24 小时在 rt 可能

Cy™ is a registered trademark of Cytiva in some countries.