Alkyne dT phosphoramidite

  • Products
  • DNA
  • Phosphoramidites
  • Alkyne dT phosphoramidite

Alkyne dT phosphoramidite

货号 规格 价格 货期
1897-100mg 100 mg 330.00$ in stock
1897-1g 1 g 2590.00$ in stock
1897-5g 5 g 9900.00$ in stock
This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. This allows to prepare modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain, by virtue of copper catalyzed Click chemistry reaction with various azides.

This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.

该试剂设计用于合成在链中间带有三键(炔烃)的寡核苷酸。 炔烃与脱氧尿苷片段相连,不会干扰与互补链的杂交。 这允许通过铜催化的与各种叠氮化物的点击化学反应制备带有荧光染料、猝灭剂和其他连接到链中间的片段的修饰寡核苷酸探针。
这种亚磷酰胺是固体,因此易于分配和处理。 它与标准解块条件兼容。

Alkyne dT phosphoramidite相关产品

Alkyne Phosphoramidite, 5′-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.


Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.

用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。

FAM-dT phosphoramidite

Phosphoramidite for synthesis of oligonucleotides labeled with fluorescein (FAM) in the middle of sequence.

亚磷酰胺用于合成在序列中间用荧光素 (FAM) 标记的寡核苷酸。

General properties

Appearance: off white solid 灰白色固体
Mass spec M+ increment: 437.1
Molecular weight: 877.96
Molecular formula: C48H56N5O9P
Solubility: good in acetonitrile, DCM
Quality control: NMR 1H, NMR 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 干燥。

MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile 乙腈
Coupling conditions: standard coupling, identical to normal nucleobases


Cleavage conditions: ammonia, 2 h at room temperature

氨水,室温下 2 小时

Deprotection conditions: identical to protected nucleobases