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  • Sulfo-Cyanine5-PEG3-Biotin


标签:sulfo-Cy5,Auxiliary reagents
Cat. # Quantity Price Lead time
2449-1mg 1 mg 120.00$ in stock

2449-5mg 5 mg 290.00$ in stock

Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.

Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.

Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.

The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.

Sulfo-Cyanine5 是一种远红荧光团,广泛用于生物分子标记,最大激发波长为 646 nm,最大发射波长为 662 nm。激发波长高于 600 nm 且发射波长超过 650 nm 的远红荧光标签对成像技术很有价值,因为这些波长的背景自发荧光较低。此外,远红荧光标签可以与近红、橙、绿、蓝标签同时成像,有利于多色成像。

众所周知,生物素或水溶性维生素 H 不仅在各种关键的代谢细胞反应中发挥重要作用,而且还因其对抗生物素蛋白(一种蛋清的糖蛋白)具有极高的亲和力。抗生物素蛋白、链霉抗生物素蛋白(抗生物素蛋白的细菌类似物)和中性抗生物素蛋白(去糖基化的抗生物素蛋白)以高亲和力与生物素非协同结合。因此,磺基-Cyanine5 生物素偶联物可用于检测和量化不同来源样品中抗生物素蛋白、链霉抗生物素蛋白和中性抗生物素蛋白的生物素结合位点。

Sulfo-Cyanine5 生物素偶联物是一种水溶性试剂,其荧光在 pH 4 到 pH 10 之间不受 pH 影响。生物素部分和荧光标签之间的灵活 PEG3 接头可与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合,而不会出现空间问题。

磺基-Cyanine5 生物素偶联物的主要应用包括成像(例如用于蛋白质印迹、免疫测定、细胞和组织化学、流式细胞术的一抗和二抗标记)、亲和力和解离常数测量、基于链霉亲和素的传感器等。

Absorption and emission spectra of sulfo-Cyanine5



Sulfo-Cyanine5 tetrazine

Water soluble Cyanine dye tetrazine derivative for TCO ligation with trans-cyclooctenes and other strained olefines.

水溶性花青染料四嗪衍生物,用于与反式环辛烯和其他应变烯烃的 TCO 连接。

dsGreen Gel Staining Solution, 10000×

dsGreen for staining dsDNA in gels.

dsGreen 用于在凝胶中染色 dsDNA。

Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

用于 Click Chemistry 的水溶性磺基-Cy3 染料叠氮化物。

General properties

Appearance: dark blue solid 深蓝色固体
Molecular weight: 1037.36
Molecular formula: C48H65N6KO11S3
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04