FAM hydrazide, 5-isomer

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  • FAM hydrazide, 5-isomer

FAM hydrazide, 5-isomer

标签:FAM (Fluorescein), Hydrazides
货号 规格 价格 货期
14170 1 mg 110.00$ in stock
24170 5 mg 210.00$ in stock
44170 25 mg 410.00$ in stock
54170 50 mg 695.00$ in stock
64170 100 mg 1190.00$ in stock

Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.

许多天然和合成分子含有醛或酮羰基。 这些羰基与酰肼反应形成腙。 该反应在中性附近的 pH 值下是自发的,生成的腙非常稳定。

含有 1,2-二醇官能团的化合物,如糖,可以用高碘酸钠氧化,形成羰基化合物,然后用酰肼修饰。 这是标记糖蛋白(如抗体)和多糖的有效方法。

FAM hydrazide 是一种酰肼标记物,用于将荧光素(一种 488 nm 通道的明亮染料)连接到羰基化合物上。

Absorption and emission spectra of FAM

FAM hydrazide, 5-isomer

FAM hydrazide, 5-isomer

FAM alkyne, 5-isomer

FAM alkyne, 5-isomer – an alkyne derivative of fluorescein dye for Click Chemistry.

FAM 炔烃,5 异构体 – 用于 Click Chemistry 的荧光素染料的炔烃衍生物。

Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.

炔烃 NHS 酯,用于标记具有炔烃基团的生物分子,用于铜催化的点击化学。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

General properties

Appearance: yellow solid 黄色固体
Mass spec M+ increment: 372.07
Molecular weight: 426.81
CAS number: 2183440-64-2
Molecular formula: C21H15N2ClO6
IUPAC name: Spiro[isobenzofuran-​1(3H)​,​9′-​[9H]​xanthene]​-​5-​carboxylic acid, 3′,​6′-​dihydroxy-​3-​oxo-​, hydrazide, hydrochloride
Solubility: good in ethanol, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17