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• FAM hydrazide, 5-isomer

FAM hydrazide, 5-isomer

Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.

Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.

FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.

许多天然和合成分子含有醛或酮羰基。 这些羰基与酰肼反应形成腙。 该反应在中性附近的 pH 值下是自发的，生成的腙非常稳定。

含有 1,2-二醇官能团的化合物，如糖，可以用高碘酸钠氧化，形成羰基化合物，然后用酰肼修饰。 这是标记糖蛋白（如抗体）和多糖的有效方法。

FAM hydrazide 是一种酰肼标记物，用于将荧光素（一种 488 nm 通道的明亮染料）连接到羰基化合物上。