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• Cyanine5.5 hydrazide

Cyanine5.5 hydrazide

Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.

Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine5.5 hydrazide to form Cyanine5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies.

Cyanine5.5 is a near infrared fluorophore, an analog of Cy5.5®. It is particularly suitable for the applications which require low fluorescence background. The absorption and emission wavelength of the dye exhibit significant tissue penetration, so Cyanine5.5 is suitable for in vivo NIR imaging applications.

Cy5.5 hydrazide 是一种羰基反应性近红外染料。酰肼与醛和酮等羰基化合物快速反应，形成稳定的腙。该反应可用于制备标记的偶联物。

生物分子中的羰基可以在氧化应激或蛋白质脱氨基后自发形成。或者，醛基可以通过糖蛋白（例如抗体）的糖残基的高碘酸氧化特异性地产生。抗体可被高碘酸钠氧化，并用Cyanine5.5酰肼标记，形成Cyanine5.5标记的抗体。由于抗体中的糖残基远离可变的表位结合位点，因此该方法特别适用于标记抗体。

Cy5.5 是一种近红外荧光团，是 Cy5.5® 的类似物。它特别适用于需要低荧光背景的应用。染料的吸收和发射波长表现出显着的组织穿透性，因此 Cy5.5 适用于体内近红外成像应用。