TAMRA maleimide, 6-isomer

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TAMRA maleimide, 6-isomer

标签:Maleimides, TAMRA (TMR, tetramethylrhodamine)
货号 规格 价格 货期
18180 1 mg 110.00$ in stock
28180 5 mg 210.00$ in stock
48180 25 mg 410.00$ in stock
58180 50 mg 695.00$ in stock
68180 100 mg 1190.00$ in stock

TAMRA (also known as TMR or tetramethylrhodamine) is a xanthene dye that has been used as a fluorescent label for decades. Xanthene dyes are available as two isomers (called 5- and 6-isomers) that have almost identical fluorescent properties, but need to be separated to avoid doubling and smearing of labeled product peaks or bands during chromatography or electrophoresis. This is a pure 6-isomer of TAMRA maleimide, used for the labeling of proteins and peptides via thiol (SH) groups.

TAMRA(也称为 TMR 或四甲基罗丹明)是一种呫吨染料,几十年来一直被用作荧光标记。 呫吨染料有两种异构体(称为 5 异构体和 6 异构体),它们具有几乎相同的荧光特性,但需要分离以避免在色谱或电泳过程中标记的产物峰或带加倍和拖尾。 这是 TAMRA 马来酰亚胺的纯 6 异构体,用于通过硫醇 (SH) 基团标记蛋白质和肽。

Absorption and emission spectra of 6-TAMRA

TAMRA maleimide, 6-isomer

TAMRA maleimide, 6-isomer相关产品

BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.

BDP R6G 是一种硼二吡咯亚甲基染料,其吸收和发射光谱与 R6G 相似。

TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.

TAMRA 染料的胺反应活性 N-羟基琥珀酰亚胺酯,用于标记蛋白质、肽和氨基寡核苷酸的胺基。

Cyanine7 hydrazide

Carbonyl reactive NIR fluorophore for the labeling of aldehydes, ketones, including glycoproteins subjected to periodate oxidation.

羰基反应性 NIR 荧光团,用于标记醛、酮,包括经过高碘酸盐氧化的糖蛋白。

General properties

Appearance: dark colored solid 深色固体
Mass spec M+ increment: 551.2
Molecular weight: 552.58
Molecular formula: C31H28N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 541
ε, L⋅mol−1⋅cm−1: 84000
Emission maximum, nm: 567
Fluorescence quantum yield: 0.1
CF260: 0.32
CF280: 0.19

相关文献:

  1. Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.M Koszegi, Z.; Bacon, A.; Trapp, S.; Jones, B.J.; Hastoy, B.; Tomas, A.; Reissaus, C.; Linnemann, A.K.; D’Este, E.; Calebiro, D.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. LUXendins reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. bioRxiv, preprint. doi: 10.1101/557132
  2. Sagert, L.; Hennig, F.; Thomas, C.; Tampé, R. A loop structure allows TAPBPR to exert its dual function as MHC I chaperone and peptide editor. eLife, 2020, 9, e55326. doi: 10.7554/eLife.55326
  3. Ast, J.; Arvaniti, A.; Fine, N.H.F.; Nasteska, D.; Ashford, F.B.; Stamataki, Z.; Koszegi, Z.; Bacon, A.; Jones, B.J.; Lucey, M.A.; Sasaki, S.; Brierley, D.I.; Hastoy, B.; Tomas, A.; D’Agostino, G.; Reimann, F.; Lynn, F.C.; Reissaus, C.A.; Linnemann, A.K.; D’Este, E.; Calebiro, D.; Trapp, S.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D.J. Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics. Nature Communications, 2020, 11, 467. doi: 10.1038/s41467-020-14309-w