FAM maleimide, 6-isomer

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  • FAM maleimide, 6-isomer

FAM maleimide, 6-isomer

标签:FAM (Fluorescein), Maleimides, Fluorescence polarization, Microscopy
货号 规格 价格 货期
24180 5 mg in stock
44180 25 mg 160.00$ in stock
54180 50 mg 270.00$ in stock
64180 100 mg 420.00$ in stock

FAM (fluorescein) is a bright fluorophore which is compatible with various fluorescence detection instruments. FAM is a universal dye that is useful for microscopy, qPCR and many other methods as well as for FRET-based and fluorescence polarization based binding assays.

FAM maleimide is a thiol reactive dye for the labeling of proteins, peptides, and other thiolated molecules.

FAM(荧光素)是一种明亮的荧光团,可与各种荧光检测仪器兼容。 FAM 是一种通用染料,可用于显微镜检查、qPCR 和许多其他方法以及基于 FRET 和荧光偏振的结合测定。

FAM 马来酰亚胺是一种硫醇活性染料,用于标记蛋白质、肽和其他硫醇化分子。

Fluorescein (FAM) absorbance and emission spectra

FAM maleimide, 6-isomer

FAM maleimide, 6-isomer相关产品

BDP FL amine

Amino derivative of BDP FL dye, borondipyrromethene dye for FAM (fluorescein) 488 nm channel.

BDP FL 染料的氨基衍生物,用于 FAM(荧光素)488 nm 通道的硼二吡咯亚甲基染料。

Cyanine5 hydrazide

Cy5 hydrazide is a reactive dye for aldehydes and ketones. It can be used for the labeling of glycoproteins after periodate oxidation of sugar moieties.

Cy5 hydrazide是醛和酮的活性染料。 它可用于糖部分高碘酸氧化后的糖蛋白的标记。

Cyanine5.5 alkyne

Cyanine5.5 alkyne is a NIR (near infrared) dye azide for both copper-catalyzed, and copper-free Click Chemistry.

Cy5.5 炔烃是一种 NIR(近红外)染料叠氮化物,适用于铜催化和无铜点击化学。

General properties

Appearance: yellow solid 黄色固体
Mass spec M+ increment: 498.4
Molecular weight: 498.44
Molecular formula: C27H18N2O8
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

相关文献:

  1. Woodford, M.R.; Baker-Williams, A.J.; Sager, R.A.; Backe, S.J.; Blanden, A.R.; Hashmi, F.; Kancherla, P.; Gori, A.; Loiselle, D.R.; Castelli, M.; Serapian, S.A.; Colombo, G.; Haystead, T.A.; Jensen, S.M.; Stetler-Stevenson, W.G.; Loh, S.N.; Schmidt, L.S.; Linehan, W.M.; Bah, A.; Bourboulia, D.; Bratslavsky, G.; Mollapour, M. The tumor suppressor folliculin inhibits lactate dehydrogenase A and regulates the Warburg effect. Nature Structural & Molecular Biology, 2021, 28(8), 662–670. doi: 10.1038/s41594-021-00633-2
  2. Nojoumi, S.; Ma, Y.; Schwagerus, S.; Hackenberger, C.P.R.; Budisa, N. In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation. International Journal of Molecular Sciences, 2019, 20(9), 2299. doi: 10.3390/ijms20092299
  3. Sauer, F.; Klemm, T.; Kollampally, R.B.; Tessmer, I.; Nair, R.K.; Popov, N.; Kisker, C. Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities. Molecular Cell, 2019, 74(3), 421–435.e10. doi: 10.1016/j.molcel.2019.02.029
  4. Yu, C.; Tang, J.; Loredo, A.; Chen, Y.; Jung, S.Y.; Jain, A.; Gordon, A.; Xiao, H. Proximity-Induced Site-Specific Antibody Conjugation. Bioconjugate Chemistry, 2018, 29(11), 3522–3526. doi: 10.1021/acs.bioconjchem.8b00680