FAM azide, 5-isomer,FAM 叠氮化物,5-isomer

  • Products
  • Reactive dyes
  • Dye azides
  • FAM azide, 5-isomer

FAM azide, 5-isomer

标签:FAM (Fluorescein), Azides, Fluorescence polarization, Microscopy
货号 规格 价格 货期
14130 100 uL, 10 mM/DMSO 80.00$ in stock
34130 500 uL, 10 mM/DMSO 100.00$ in stock
44130 1 mL, 10 mM/DMSO 150.00$ in stock
A4130 1 mg 80.00$ in stock
B4130 5 mg 100.00$ in stock
C4130 10 mg 150.00$ in stock
D4130 25 mg 295.00$ in stock
E4130 50 mg 450.00$ in stock
F4130 100 mg 780.00$ in stock

FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent labels for various applications. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for DyLight 488.

用于点击化学标记的 FAM 叠氮化物。 FAM 仍然是各种应用中最受欢迎的荧光标记之一。 大多数能够进行荧光检测的仪器,从读板器到荧光显微镜,都能够在 FAM 通道中工作。

借助 Click 化学和该试剂的多功能性,可以将这种流行的荧光团连接到几乎任何带有炔烃的分子上。

FAM 叠氮化物既可作为固体化合物使用,也可作为 10 mM DMSO 溶液提供,可用于我们推荐的标记方案。 本品为纯5-异构体。 FAM 是 DyLight 488 的替代品。

FAM azide, 5-isomer,FAM 叠氮化物,5-isomer相关产品

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.

BDP TMR 的胺活性 NHS 酯,用于 TAMRA 通道的明亮硼二吡咯亚甲基染料,特别适用于荧光偏振测定。

HEX azide, 6-isomer

Azide of green-yellow emitting HEX dye is used for conjugation with oligonucleotides via [3+2]-dipolar cycloaddition.

绿黄色发射 HEX 染料的叠氮化物用于通过 [3+2]-偶极环加成与寡核苷酸缀合。

BDP TR azide

BDP TR is a red emitting fluorophore for ROX channel. This is an azide for copper catalyzed and copper free Click chemistry conjugation.

BDP TR 是一种用于 ROX 通道的红色发射荧光团。 这是一种用于铜催化和无铜点击化学偶联的叠氮化物。

General properties

Appearance: yellowish crystals 淡黄色晶体
Molecular weight: 458.42
CAS number: 510758-23-3
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

储存:收到后 24 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

相关文献:

  1. Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
  2. Gueiderikh, A.; Maczkowiak-Chartois, F.; Rouvet, G.; Souquère-Besse, S.; Apcher, S.; Diaz, J.-J.; Rosselli, F. Fanconi anemia A protein participates in nucleolar homeostasis maintenance and ribosome biogenesis. Science Advances, 2021, 7(1), eabb5414. doi: 10.1126/sciadv.abb5414
  3. Ramey-Ward, A.N.; Su, H.; Salaita, K. Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis. ACS Applied Materials & Interfaces, 2020, 12(32), 35903–35917. doi: 10.1021/acsami.0c08871
  4. Kim, D.; Singh, N.; Waldemer-Streyer, R.J.; Yoon, M.-S.; Chen, J. Muscle-derived TRAIL negatively regulates myogenic differentiation. Experimental Cell Research, 2020, 394(1), 112165. doi: 10.1016/j.yexcr.2020.112165