Application: ELISA, IF, WB
Puromycin is an aminonucleoside antibiotic derived from the bacterial strain Streptomyces alboniger. Structurally resembling the 3′ end of aminoacyl-tRNAs, it can bind to translating ribosomes and cause premature chain termination. Classically, protein biosynthesis has been monitored using pulse-chase and flooding dose methods which both employ radioactive amino acid labels. Analysis using puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling and allows for the direct evaluation/quantification of translation using standard immunochemical methods.
1) Easy to evaluate and quantify protein biosynthesis
2) An alternative to the traditional pulse-chase method that relies on radioactive amino acids
3) Applicable to Western blot and ELISA analyses
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