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- AF488 DBCO, 5-isomer
AF488 DBCO, 5-isomer
| Cat. # | Quantity | Price | Lead time | |
|---|---|---|---|---|
| 1118F0 | 1 mg | 110.00$
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| 2118F0 | 5 mg | 290.00$
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| 4118F0 | 25 mg | 690.00$
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| 5118F0 | 50 mg | 1270.00$
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| 6118F0 | 100 mg | 1990.00$
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
AF488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Customers also purchased with this product
FAM DBCO, 6-isomer
Cyclooctyne derivative of fluorescein (FAM) for copper free Click chemistry reaction with azides. Contains pure 6-isomer of fluorescein.
BDP FL DBCO is a dye cyclooctyne for copper free Click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
