ProSpec,CD14 Mouse,CD14 小鼠重组 PRO-537

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

CD14 receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol and functions as a high affinity receptor for complexes of LPS and LPS binding protein . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

CD14 受体）在单核细胞和巨噬细胞上强烈表达，在中性粒细胞表面表达较弱。 CD14 通过与糖基磷脂酰肌醇连接而锚定在细胞上，并作为 LPS 和 LPS 结合蛋白复合物的高亲和力受体发挥作用。 可溶性 CD14 也与 LPS 结合，在生理浓度下作为 LPS 激动剂起作用，并且在较高浓度下，在细胞活化中具有 LPS 拮抗作用。 CD14 已显示与凋亡细胞结合。

The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by page.
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

CHO-cells.

Sterile Filtered White lyophilized powder.

CD14 was lyophilized from a concentrated protein solution containing phosphate-buffered saline, pH 7.2.

It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse CD14 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

• ELISA.
• Inhibition Assays.
• Western Blotting.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.