SDF-1, CXCL12, Pre-B cell growth-stimulating factor, PBSF, hIRH, chemokine ligand 12, SDF1, SDF1A, TPAR1, SCYB12, SDF-1a, TLSF-a, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, Thymic lymphoma cell-stimulating factor, TLSF.
SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine ligand 12 . It is produced in two forms, SDF-1?/CXCL12a and SDF-1?/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes and has been implicated as an important cell co-ordinator during development. During embryogenesis it directs the migration of hematopoietic cells from foetal liver to bone marrow. Mice which were knocked-out for CXCL12 gene were lethal before the birth or within just 1 hour of life. As another role, CXCL12a alters also the electrophysiology of neurons. CXCL12 was shown to be expressend in many tissues in mice .
The receptor for this chemokine is CXCR4, which was previously called fusin. This CXCL12-CXCR4 interaction used to be considered exclusive , but recently it was suggested that CXCL12 is also bound by CXCR7 receptor.
The gene for CXCL12 is located on human chromosome 10. In human and mouse both CXCL12 and CXCR4 show high identity of sequence: 99% and 90%, respectively.
SDF-1 是属于趋化因子家族的小细胞因子，正式命名为趋化因子配体 12。它通过同一基因的交替剪接以两种形式产生，SDF-1?/CXCL12a 和 SDF-1?/CXCL12b。趋化因子的特征在于存在四个保守的半胱氨酸，它们形成两个二硫键。 CXCL12 蛋白属于 CXC 趋化因子组，其初始半胱氨酸对由一个中间氨基酸隔开。 CXCL12 对淋巴细胞具有很强的趋化性，并且在发育过程中被认为是重要的细胞协调因子。在胚胎发生过程中，它指导造血细胞从胎儿肝脏迁移到骨髓。 CXCL12 基因被敲除的小鼠在出生前或出生后仅 1 小时内都是致命的。作为另一个作用，CXCL12a 也改变了神经元的电生理。 CXCL12 显示在小鼠的许多组织中表达。
这种趋化因子的受体是 CXCR4，以前称为融合蛋白。这种 CXCL12-CXCR4 相互作用曾经被认为是排他性的，但最近有人提出 CXCL12 也与 CXCR7 受体结合。
CXCL12 的基因位于人类第 10 号染色体上。在人类和小鼠中，CXCL12 和 CXCR4 均显示出高度的序列同一性：分别为 99% 和 90%。
Stromal Cell-Derived Factor-1 alpha Mouse Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8 kDa.
The SDF-1a is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized powder.
The protein was Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid .
It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized SDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的 SDF-1a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CXCL12 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
Greater than 95% as determined by -PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
The specific activity as determined by its ability to chemoattract human peripheral blood monocytes at 50-100 ng/ml corresponding to a Specific Activity of 10,000-20,000IU/mg.
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