Catalogue number
CYT-750
Synonyms
Apolipoprotein A-I, Apo-AI, ApoA-I, APOA1, MGC117399.
Introduction
APOA1 is a human protein with a specific role in lipid metabolism being the main protein component of HDL in the plasma. APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Furthermore, APOA1 is a cofactor for LCAT, which is responsible for the formation of most plasma cholesteryl esters. In addition, APOA1 activates spermatozoa motility as part of the SPAP complex. The APOA1 gene is strongly linked with two other apolipoprotein genes on chromosome 11. Defects in the APOA1 gene are linked to HDL deficiency including Tangier disease, and with systemic non-neuropathic amyloidosis. High levels of APOA1 are linked to the manifestation of asthma and atopy.
APOA1 是一种人类蛋白质，在脂质代谢中具有特定作用，是血浆中 HDL 的主要蛋白质成分。 APOA1 促进胆固醇从组织流出到肝脏进行排泄。 此外，APOA1 是 LCAT 的辅助因子，它负责大多数血浆胆固醇酯的形成。 此外，APOA1 作为 SPAP 复合物的一部分激活精子运动。 APOA1 基因与 11 号染色体上的其他两个载脂蛋白基因密切相关。APOA1 基因的缺陷与 HDL 缺乏症（包括丹吉尔病）和系统性非神经病性淀粉样变性有关。 高水平的 APOA1 与哮喘和特应性的表现有关。
Description
Apolipoprotein A-I Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 243 amino acids and having a molecular mass of 28.1kDa.
The APOA1 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的载脂蛋白 A-I 人重组体是一条单一的、非糖基化的多肽链，包含 243 个氨基酸，分子量为 28.1kDa。
APOA1 通过专有的色谱技术进行纯化。
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The APOA1 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized APOA1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干 APOA1，然后可以进一步稀释成其他水溶液。
Stability
Lyophilized Apolipoprotein A-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的载脂蛋白 A-I 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，APOA1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。
Purity
Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.
Amino acid sequence
DEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ.
Usage
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
