ProSpec-BAFF Human, Plant/CYT-054

BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.

BAFF binds to tnfrsf13b/taci and tnfrsf17/bcma. Tnfsf13/april binds to the same 2 receptors, together, they form a 2 ligands -2 receptors pathway involved in the stimulation of b- and t-cell function and the regulation of humoral immunity.A third b-cell specific baff-receptor promotes the survival of mature b-cells and the b-cell response.
B Lymphocyte Stimulator functions as a potent B-cell growth factor in costimulation assays.
Administration of BAFF Human recombinant to mice disrupts splenic B-cell and T-cell zones and results in elevated levels of serum immunoglobulin.

BAFF 与 tnfrsf13b/taci 和 tnfrsf17/bcma 结合。 Tnfsf13/april 与相同的 2 个受体结合，共同形成 2 个配体 -2 受体通路，参与刺激 b 和 t 细胞功能和调节体液免疫。第三种 b 细胞特异性 baff 受体促进 成熟 b 细胞的存活和 b 细胞反应。
B 淋巴细胞刺激剂在共刺激试验中用作有效的 B 细胞生长因子。
给小鼠施用 BAFF Human 重组体会破坏脾 B 细胞和 T 细胞区域，并导致血清免疫球蛋白水平升高。

BAFF human Recombinant produced in Nicotiana benthamiana plant is a single glycosilated polypeptide chain containing 151 amino acids fragment .
BAFF is fused to a 10-His-tag at the N-terminal having the total molecular mass of 18-20kDa and purified by standard chromatographic techniques.

在本氏烟草植物中产生的 BAFF 人重组体是含有 151 个氨基酸片段的单糖基化多肽链。
BAFF 在 N 末端与总分子量为 18-20kDa 的 10-His-标签融合，并通过标准色谱技术进行纯化。

Nicotiana benthamiana plant

Sterile Filtered White lyophilized powder.

Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7 and 0.2 M NaCl.

It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 BAFF 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中，然后可以进一步稀释成其他水溶液。

Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 BAFF 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，BAFF 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by Analysis by -PAGE.

HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL

The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED50 ? 50ng/ml

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。