ProSpec-GDF3 Human/CYT-694,Growth Differentiation Factor-3 Human Recombinant

Growth Differentiation Factor 3, Growth/Differentiation Factor 3 , MCOPCB6, MCOP7, GDF-3, KFS3.

GDF3 is a member of the TGF-beta superfamily though it does not show similarity pattern of conserved cysteine residues. GDF3 is linked to Vg-1 and human BMP-4. GDF3 transcripts are identified mainly in adult bone marrow, spleen, thymus, and adipose tissue. GDF3 expression is upregulated strongly in high-fat-fed C57Bl/6J FABP4/aP2 null mice, which develop obesity but not the related hyperglycemia or hyperinsulinemia characteristic of type II diabetes. GDF3 expression therefore bonds fatty acid metabolism in adipocytes and the expression of a differentiation regulator belonging to the bone morphogenetic proteins.

GDF3 是 TGF-β 超家族的成员，尽管它不显示保守半胱氨酸残基的相似模式。 GDF3 与 Vg-1 和人类 BMP-4 相关联。 GDF3 转录物主要在成人骨髓、脾脏、胸腺和脂肪组织中被发现。 GDF3 表达在高脂喂养的 C57Bl/6J FABP4/aP2 缺失小鼠中强烈上调，这些小鼠会出现肥胖，但不会出现 II 型糖尿病的相关高血糖或高胰岛素血症特征。 因此，GDF3 表达结合了脂肪细胞中的脂肪酸代谢和属于骨形态发生蛋白的分化调节因子的表达。

GDF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 124 amino acids and having a total molecular mass of 14.15 kDa.
GDF3 is fused to a 10 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 GDF3 Human Recombinant 是单一的、非糖基化的多肽链，包含 124 个氨基酸，总分子量为 14.15 kDa。
GDF3 在 N 末端与 10 个氨基酸的 His 标签融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Filtered White lyophilized powder.

The protein was lyophilized from a concentrated solution containing 30mM Acetate buffer pH-4.

It is recommended to reconstitute the lyophilized GDF3 in sterile 100mM Acetate buffer pH-4 at a concentration of 0.5mg/ml. For the dilution into higher pH values, it is recommended to dilute the protein to a concentration of 10μg/ml. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not sterile! Please sterile filter before using it in the cell culture.

建议在无菌 100mM 醋酸缓冲液 pH-4 中以 0.5mg/ml 的浓度重构冻干的 GDF3。 为了稀释到更高的 pH 值，建议将蛋白质稀释到 10μg/ml 的浓度。 请注意，在较高浓度下，GDF3 的溶解度是有限的。 蛋白质不是无菌的！ 在细胞培养中使用前请无菌过滤。

Lyophilized GDF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF3 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 GDF3 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，GDF3 应在 4°C 下储存 2-7 天，以备将来使用
-18°C。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 90.0% as determined by -PAGE.

MKHHHHHHAS AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDECGCG.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。