ProSpec-CTF1 Mouse/CYT-151

CTF1, CT1, CT-1, Cardiophin 1.

Cardiotrophin 1 is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.
CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins in cardiac cells.
Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide and angiotensinogen mRNA.
Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.

心肌营养素 1 是白细胞介素 6 超家族的 201 个氨基酸成员。它是通过其在心肌细胞中诱导肥大反应的能力来确定的。 CT-1 mRNA 水平在心肌细胞和心脏非心肌细胞中均存在。 CT 1 在正常成人肺中也大量检测到，并在胎儿和成人气道平滑肌细胞中表达。 CT 1 激活 gp130 依赖性信号传导并刺激 Janus 激酶/信号转导和转录途径激活因子，以转导心肌细胞中的肥大和细胞保护信号。
CT 1 还具有神经营养功能。在胚胎第 14 天和出生后第一周之间，CTF1 缺乏导致小鼠脊髓和脑干核中运动神经元细胞死亡增加。此外，CT-1 是一种肝细胞存活因子，可有效减少急性肝损伤动物模型中的肝细胞损伤。心肌营养因子 1 的表达在缺氧刺激后增强，当在模拟缺血之前或在模拟缺血后的复氧时添加时，它可以保护心肌细胞。 Cardiotrophin 1 可以诱导心脏细胞中保护性热休克蛋白的表达。
Cardiotrophin-1 增加 ANP、脑利钠肽和血管紧张素原 mRNA 的心室表达。
Cardiophin 1 水平在心力衰竭患者、扩张型心肌病患者、中/重度二尖瓣关闭不全、稳定和不稳定型心绞痛患者以及急性心肌梗死后显着升高。

CTF1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids and having a molecular mass of 21.3kDa.
The CTF1 Mouse is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 CTF1 小鼠重组体是一条单一的、非糖基化的多肽链，含有 202 个氨基酸，分子量为 21.3kDa。
CTF1 Mouse 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl to a concentration of 0.1-0.5 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20⁰C. Further dilutions should be made in appropriate buffered solutions.

Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的 CTF1 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，CTF1 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 98.0% as determined by -PAGE.

SQREGSLEDH QTDSSISFLP HLEAKIRQTH NLARLLTKYA EQLLEEYVQQ QGEPFGLPGF SPPRLPLAGL SGPAPSHAGL PVSERLRQDA AALSVLPALL DAVRRRQAEL NPRAPRLLRS LEDAARQVRA LGAAVETVLA ALGAAARGPG PEPVTVATLF TANSTAGIFS AKVLGFHVCG LYGEWVSRTE GDLGQLVPGG VA

The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 1.0ng/ml, corresponding to a specific activity of > 1,000,000units/mg.

由 TF-1 细胞的剂量依赖性增殖确定的 ED50 < 1.0ng/ml，对应于 > 1,000,000units/mg 的比活性。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.