ProSpec-CTLA4 Human, Sf9/CYT-952

Cytotoxic T-Lymphocyte Associated Protein 4, Cytotoxic T-Lymphocyte-Associated Protein 4, Insulin-Dependent Diabetes Mellitus 12, Celiac Disease 3, CTLA-4, CD152, Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4, Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form, Cytotoxic T-Lymphocyte-Associated Serine Esterase-4, Cytotoxic T-Lymphocyte-Associated Antigen 4, CD152 Isoform, CD152 Antigen, CELIAC3, IDDM12, ALPS5, GRD4, GSE, CD, Cytotoxic T-lymphocyte protein 4, CTLA4.

CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.

CTLA-4 是免疫球蛋白超家族的成员，编码一种将抑制信号传递给 T 细胞的蛋白质。 该蛋白质包含一个V结构域、一个跨膜结构域和一个胞质尾。 已经表征了编码不同同种型的替代转录剪接变体。 膜结合异构体作为通过二硫键互连的同二聚体发挥作用，而可溶异构体作为单体发挥作用。 该基因的突变与胰岛素依赖型糖尿病、格雷夫斯病、桥本甲状腺炎、乳糜泻、系统性红斑狼疮、甲状腺相关眼眶病和其他自身免疫性疾病有关。

CTLA4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 14.6kDa .
CTLA4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

在 Sf9 杆状病毒细胞中产生的 CTLA4 是单条糖基化多肽链，含有 135 个氨基酸，分子量为 14.6kDa。
CTLA4 在 C 末端用 6 个氨基酸 His 标签表达，并通过专有色谱技术进行纯化。

Sf9, Baculovirus cells.

Sterile filtered colorless solution.

CTLA4 protein solution contains Phosphate Buffered Saline and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

Greater than 90% as determined by -PAGE.

Determined by IL-2 ELISA via Jurkat human acute T cell leukemia cells. The ED50 range ≤ 150 ng/ml with Human B7-1/CD80.

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ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.