ProSpec-CTLA4 Mouse/CYT-950

Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, Ctla4, Cd152.

CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with ins.-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.

CTLA-4 是免疫球蛋白超家族的成员，编码一种将抑制信号传递给 T 细胞的蛋白质。 该蛋白质包含一个V结构域、一个跨膜结构域和一个胞质尾。 已经表征了编码不同同种型的替代转录剪接变体。 膜结合异构体作为通过二硫键互连的同二聚体发挥作用，而可溶异构体作为单体发挥作用。 该基因的突变与依赖于胰岛素的糖尿病、格雷夫斯病、桥本甲状腺炎、乳糜泻、系统性红斑狼疮、甲状腺相关眼眶病和其他自身免疫性疾病有关。

CTLA4 Mouse Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 363 amino acids and having a molecular mass of 40.6kDa .
CTLA4 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.

在 Sf9 昆虫细胞中产生的 CTLA4 Mouse Recombinant 是单条糖基化多肽链，含有 363 个氨基酸，分子量为 40.6kDa。
CTLA4 在 C 末端与 239 个氨基酸的 hIgG-His-tag 融合，并通过专有色谱技术进行纯化。

Sf9 Insect cells.

Sterile filtered colorless solution.

CTLA4 protein solution contains Phosphate Buffered Saline and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90.0% as determined by -PAGE.

The ED50 range ≤150ng/ml and the activity is determined by the IL-2 ELISA in a using stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. 

ED50 范围≤150ng/ml，活性由 IL-2 ELISA 测定，使用刺激的 Jurkat 人急性 T 细胞白血病细胞和人 B7-1/CD80。

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PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH
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ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.