ProSpec-EREG Human/CYT-609

EREG, Epiregulin, ER.

Epiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB family of tyrosine-kinase receptors.Epiregulin is expressed mostly in the placenta and peripheral blood leukocytes and in specific carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. Epiregulin inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.

上皮调节蛋白是 EGF 家族的一部分。 上皮调节蛋白作为 EGFR 的配体，以及酪氨酸激酶受体 ERBB 家族大多数成员的配体。上皮调节蛋白主要在胎盘和外周血白细胞以及膀胱、肺、肾和结肠的特定癌中表达 . 上皮调节蛋白刺激角质形成细胞、肝细胞、成纤维细胞和血管平滑肌细胞的增殖。 Epiregulin 抑制几种肿瘤来源的上皮细胞系的生长。 人上皮调节蛋白最初合成为糖基化的 19.0 kDa 跨膜前体蛋白，通过蛋白水解切割加工产生 6.0 kDa 成熟分泌序列。

Epiregulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 49 amino acids and having a molecular mass of 5.6 kDa.
Epiregulin is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 Epiregulin Human Recombinant 是单一的、非糖基化的多肽链，包含 49 个氨基酸，分子量为 5.6 kDa。
上皮调节蛋白通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Epiregulin was lyophilized from 0.5mg/ml solution ciontaing 20mM PBS buffer pH-7.4 containing 20mM sodium chloride.

It is recommended to reconstitute the lyophilized Epiregulin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的上皮调节蛋白在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶，然后可以进一步稀释成其他水溶液。

Lyophilized Epiregulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Epiregulin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的上皮调节蛋白虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，上皮调节蛋白应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is < 2.0 ng/ml, corresponding to a specific activity of > 500,000 units/mg.

通过对鼠 Balb/3T3 细胞增殖的剂量依赖性刺激确定 ED50 < 2.0 ng/ml，对应于 > 500,000 单位/mg 的比活性。

Greater than 97.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

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ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.