ProSpec-SHH Mouse, His/CYT-711

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

重组人声波刺猬是一小部分分泌蛋白的一部分，这些蛋白对脊椎动物和无脊椎动物的发育都至关重要。 3个哺乳动物刺猬基因具有约60%的同源性。人类声波刺猬与小鼠基因有 99% 的同源性。 Sonic HedgeHog 是一种对引导早期胚胎至关重要的蛋白质。它被认为是腹侧神经管、前后肢轴和腹侧体节的主要诱导信号。 Sonic HedgeHog 与修补受体结合，该受体与 Smoothened 相关联，以激活靶基因的转录。在没有声波 HedgeHog 的情况下，修补受体抑制了平滑的本构信号活性。 Sonic HedgeHog 还调节另一个因素，即 gli 癌基因。 Sonic HedgeHog 细胞间信号对于发育过程中的各种模式事件至关重要：由脊索产生的信号，该信号诱导神经管和体节中的腹侧细胞命运，以及用于形成发育中的肢芽前后轴模式的极化信号。 Sonic HedgeHog 表现出底板和运动神经元诱导活动。位于肢体区域 1 基因内含子的远程 Sonic HedgeHog 增强子的突变导致轴前多指畸形。

SHH Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 183 amino acids and having a molecular mass of 20.8 kDa. SHH protein is fused to a 8 amino acid His-Tag at C-terminus and purified by standard chromatography.

在大肠杆菌中产生的 SHH Mouse Recombinant 是一条单一的、非糖基化的多肽链，含有 183 个氨基酸，分子量为 20.8 kDa。 SHH 蛋白在 C 末端与 8 个氨基酸的 His-Tag 融合，并通过标准色谱法纯化。

Escherichia Coli.

Sterile filtered colorless solution.

SHH Mouse His Tag solution containing 20mM Trsi HCL pH-8, 1mM DTT, and 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein . Please prevent freeze thaw cycles.

Greater than 95% as determined by -PAGE.

MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.