ProSpec-IFN a 1b Human/CYT-283

IFN a 1b Human

Interferon-Alpha 1b Human Recombinant
CYT-283
ProSpec-IFN a 1b Human/CYT-283 20µg
ProSpec-IFN a 1b Human/CYT-283 100µg
ProSpec-IFN a 1b Human/CYT-283 1mg

Catalogue number

CYT-283

Introduction

IFN-alpha 1b is produced by macrophages, IFN-alpha 1b has antiviral activities and stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
IFN-α1b 由巨噬细胞产生,IFN-α1b 具有抗病毒活性并刺激两种酶的产生:蛋白激酶和寡腺苷酸合成酶。

Description

Interferon-alpha 1b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton.
The Interferon-alpha 1b gene was obtained from human leukocytes.
The IFN-a 1b is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的干扰素-α 1b 人重组体是一种单一的、非糖基化的多肽链,含有 166 个氨基酸,分子量为 19392 道尔顿。
干扰素-α 1b 基因是从人类白细胞中获得的。
IFN-a 1b 通过专有色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4, containing 4% mannitol and 1% HSA.

Solubility

It is recommended to reconstitute the lyophilized IFN alpha 1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Interferon alpha 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 1b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的干扰素 α1b 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重构后,IFN-α 1b 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Biological Activity

The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.

Purity

Greater than 96.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.

确定了前五个 N 端氨基酸的序列,发现是 Cys-Asp-Leu-Pro-Glu。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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