ProSpec-IFN a 2a Human, Plant/CYT-543

Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.

IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.

IFN-α 由巨噬细胞产生并具有抗病毒活性。 干扰素刺激两种酶的产生：蛋白激酶和寡腺苷酸合成酶。

Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa.
The Interferon-a 2a contains affinity 6xHis tag on C-terminus.
The IFN-A 2a is purified by proprietary chromatographic techniques.

植物中产生的干扰素 Alpha Human 2a Recombinant 是单一的糖基化多肽链，包含 165 个氨基酸，分子量为 19 kDa。
干扰素-a 2a 在 C 末端含有亲和力 6xHis 标签。
IFN-A 2a 通过专有色谱技术进行纯化。

Nicotiana Sp.Plant.

Sterile Filtered White lyophilized powder.

Lyophilized, containing 50% .

It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的干扰素 α2a 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，IFN-α 2a 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.

Greater than 99.0% as determined by both:
Analysis by RP-HPLC.
Analysis by -PAGE.

The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 × 10⁸ Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.

比活性通过使用人 I 型干扰素敏感细胞的定量基因报告生物测定来确定。 与在相同条件下测定的细菌来源的重组 IFN-α 2a 相比，植物来源的 IFN-α 2a 在参考使用牛肾 MDBK 细胞的病毒抗性测定时显示为 6.1 × 108 单位/mg。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.