ProSpec-IFN a 2b Human, Yeast/CYT-460

IFN a 2b Human, Yeast

Interferon-Alpha 2b Human Recombinant, Yeast
CYT-460
ProSpec-IFN a 2b Human, Yeast/CYT-460 10µg
ProSpec-IFN a 2b Human, Yeast/CYT-460 50µg
ProSpec-IFN a 2b Human, Yeast/CYT-460 1mg

Catalogue number

CYT-460

Synonyms

Interferon alpha 2b, IFNA, INFA2, IFN-? 2b, MGC125764, MGC125765.

Introduction

IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.
IFN-α 由巨噬细胞产生并具有抗病毒活性。 干扰素刺激两种酶的产生:蛋白激酶和寡腺苷酸合成酶。

Description

Interferon-alpha 2b Human Recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa.
The Interferon-alpha 2b gene was obtained from human leukocytes.
The IFN-a 2b is purified by proprietary chromatographic techniques.
酵母中产生的干扰素-α 2b 人重组体是单条糖基化多肽链,含有 165 个氨基酸,分子量约为 19 kDa。
干扰素-α 2b 基因是从人类白细胞中获得的。
IFN-a 2b 通过专有色谱技术进行纯化。

Source

Saccharomyces cerevisiae.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重构冻干的糖基化 IFN α2b,然后可以进一步稀释成其他水溶液。

Stability

Lyophilized glycosilated IFN-a 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
冻干的糖基化 IFN-a 2b 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重构后,IFN-α 2b 应在 4°C 下储存 2-7 天,以备将来在低于 -18°C 的温度下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 98.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln.
确定了前五个 N 端氨基酸的序列,发现是 Cys-Asp-Leu-Pro-Gln。

Biological Activity

The specific activity as determined in a viral resistance assay was found to be no less than 300,000,000IU/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec 的产品仅供实验室研究使用。 它们不得用作药物、农业或农药产品、食品添加剂或家用化学品。

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