ProSpec-IFN g Rat/CYT-359

IFN g Rat

IFN-Gamma Rat Recombinant
CYT-359
ProSpec-IFN g Rat/CYT-359 20µg
ProSpec-IFN g Rat/CYT-359 100µg
ProSpec-IFN g Rat/CYT-359 1mg

Catalogue number

CYT-359

Synonyms

Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.

Introduction

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I IFNs.

由特定抗原或有丝分裂原激活的淋巴细胞产生的 IFN-γ。
IFN-γ除具有抗病毒活性外,还具有重要的免疫调节功能,是巨噬细胞的强效激活剂,对转化细胞具有抗增殖作用,并能增强I型干扰素的抗病毒和抗肿瘤作用。

Description

IFN-gamma Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 135 amino acids and having a molecular mass of 15609 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 IFN-γ 大鼠重组体是一种单一的、非糖基化的多肽链,含有 135 个氨基酸,分子量为 15609 道尔顿。
IFN-γ 通过专有的色谱技术进行纯化。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized IFN-gamma in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 IFN-γ 虽然在室温下可稳定保存 3 周,但应在 -18°C 以下干燥储存。 重构后,IFN-γ 应在 4°C 下储存 2-7 天,以备将来在 -18°C 以下使用。
对于长期储存,建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Gly-Tyr-Leu.

Biological Activity

The specific activity as determined by the cytopathic affect inhibition assay with murine L929 cells chalenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of 10,000,000units/mg.

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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