ProSpec-LIF Human, His/CYT-1082

CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

白血病抑制因子也称为 LIF，是一种淋巴因子，通过抑制自发分化来促进胚胎干细胞的长期维持。 白血病抑制因子具有多种功能，例如胆碱能神经元分化、干细胞多能性控制、骨和脂肪代谢、因子依赖性细胞系的有丝分裂和促进体内巨核细胞生成。 人和小鼠 LIF 在其氨基酸序列中表现出 78% 的同一性。

LIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 189 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 20.9kDa .

在大肠杆菌中产生的 LIF Human Recombinant 是一个单一的、非糖基化的多肽链，含有 189 个氨基酸，包括一个 9 个氨基酸的 N 端 His 标签。 总分子量为20.9kDa。

Escherichia Coli.

Filtered White lyophilized powder.

LIF filtered and lyophilized from 0.5mg/ml solution in 20 mM Tris buffer, 20 mM NaCl and 5% w/v trehalose, pH 7.5.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

建议加入去离子水配制约 0.5mg/ml 的工作原液，让冻干颗粒完全溶解。

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

将冻干蛋白储存在 -20°C。 重组后分装产品以避免重复的冷冻/解冻循环。 重组蛋白可在 4°C 下保存一段时间； 在 4°C 下两周后它没有显示任何变化。

Greater than 95.0% as determined by -PAGE.

MKHHHHHHAS PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF.

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