ProSpec-LIF Human, Yeast/CYT-191

CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

白血病抑制因子也称为 LIF，是一种淋巴因子，通过抑制自发分化来促进胚胎干细胞的长期维持。 白血病抑制因子具有多种功能，例如胆碱能神经元分化、干细胞多能性控制、骨和脂肪代谢、因子依赖性细胞系的有丝分裂和促进体内巨核细胞生成。 人和小鼠 LIF 在其氨基酸序列中表现出 78% 的同一性。

LIF Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 58.5 kDa. The LIF is purified by proprietary chromatographic techniques.

在酵母中产生的 LIF Human Recombinant 是单条糖基化多肽链，包含 180 个氨基酸，分子量为 58.5 kDa。 LIF 通过专有的色谱技术进行纯化。

Pichia pastoris.

Sterile Filtered White lyophilized powder.

The protein was lyophilized from a 0.2 µm filtered PBS.

It is recommended to reconstitute the lyophilized LIF in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

冻干的 LIF 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 复溶后，LIF 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

The biological activity of recombinant human LIF was measured by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimal detectable concentration of human LIF in this assay is <0.05 ng/mL. The specific activity is > 1 x 10⁸ units/mg.

S P L P I T P V N A T C A I R H P C H N N L M N Q I R S Q L A Q L N G S A N A L F I L Y Y T A Q G E P F P N N L D K L C G P N V T D F P P F H A N G T E K A K L V E L Y R I V V Y L G T S L G N I T R D Q K I L N P S A L S L H S K L N A T A D I L R G L L S N V L C R L C S K Y H V G H V D V T Y G P D T S G K D V F Q K K K L G C Q L L G K Y K Q I I A V L A Q A F.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。