ProSpec-LIF Mouse/CYT-645

CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA.

Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.

白血病抑制因子也称为 LIF，是一种淋巴因子，通过抑制自发分化来促进胚胎干细胞的长期维持。 白血病抑制因子具有多种功能，例如胆碱能神经元分化、干细胞多能性控制、骨和脂肪代谢、因子依赖性细胞系的有丝分裂和促进体内巨核细胞生成。 人和小鼠 LIF 在其氨基酸序列中表现出 78% 的同一性。

Leukemia Inhibitory Factor Murine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa.
The Leukemia Inhibitory Factor is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的白血病抑制因子鼠重组物是一种单一的、非糖基化的多肽链，含有 181 个氨基酸，分子量为 20 kDa。
白血病抑制因子通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Leukemia Inhibitory Factor was lyophilized from a concentrated sterile solution containing 20mM Phosphate buffer pH-7.4 and 0.02% Tween-20.

It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的白血病抑制因子在不低于 100μg/ml 的无菌水中复溶，然后可以进一步稀释成其他水溶液。

Lyophilized Leukemia Inhibitory Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的白血病抑制因子虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重构后，白血病抑制因子应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

MSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFP NNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNP TAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQR KKLGCQLLGTYKQVISVVVQAF.

Activity of murine LIF was determined by the M1 cell differentiation assay which was found to be < 0.01 ng/ml, corresponding to a specific activity of 100,000,000 IU/mg.
A standard of 50 Units is defined as the concentration of mouse LIF in 1.0 mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.

通过 M1 细胞分化试验确定鼠 LIF 的活性，发现其 < 0.01 ng/ml，对应于 100,000,000 IU/mg 的比活性。
50 单位的标准定义为 1.0 mL 组织培养基中小鼠 LIF 的浓度，该浓度可诱导 50% 的 M1 菌落分化。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.