ProSpec-TNF b Human/CYT-224,Tumor Necrosis Factor-Beta Human Recombinant

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

Lymphotoxin alpha 是肿瘤坏死因子家族的成员，是一种由淋巴细胞产生的细胞因子。 LTA 具有高度诱导性、分泌性，并以同源三聚体分子的形式存在。 LTA 与淋巴毒素-β 形成异源三聚体，将淋巴毒素-α 锚定到细胞表面。 LTA 介导多种炎症、免疫刺激和抗病毒反应。 LTA 还参与发育过程中次级淋巴器官的形成，并在细胞凋亡中发挥作用。

Tumor Necrosis Factor-b Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton.
The TNF-b is purified by standard chromatographic techniques.

在大肠杆菌中产生的肿瘤坏死因子-b 人重组体是一种单一的、非糖基化的多肽链，含有 172 个氨基酸，分子量为 18645 道尔顿。
TNF-b通过标准色谱技术纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized protein with no additives.

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的肿瘤坏死因子-b 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后 TNF-b 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAG.

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard.

蛋白质定量通过两种独立的方法进行：
1. 280 nm 的紫外光谱，使用 1.082 的吸光度值作为 0.1% 溶液的消光系数。 该值是由 PC GENE 计算机蛋白质序列分析程序计算得出的。
2. 通过 RP-HPLC 分析，使用 TNF-b 的校准溶液作为参考标准。

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.