ProSpec-TNF b Human, His/CYT-495,Tumor Necrosis Factor-Beta Human Recombinant, His Tag

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

TNF-b is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.

TNF-b 是一种有效的多功能细胞因子，主要由活化的 T 和 B 淋巴细胞产生。 该蛋白质已被证明对肿瘤细胞和多种其他靶细胞具有特定的有效细胞毒活性，并且是炎症和免疫功能的介质。

TNF-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids and having a molecular mass of 20.9kDa.
TNF-b is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNF-b Human Recombinant 是一种单一的、非糖基化的多肽链，含有 192 个氨基酸，分子量为 20.9kDa。
TNF-b 在 N 端与 21 个氨基酸的 His-tag 融合，并通过专有色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered clear solution.

TNF-b protein solution contains 20mM Tris-HCl buffer , 30% glycerol, 0.1M NaCl and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein .
Avoid multiple freeze-thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。 储存，在 -20°C 下冷冻更长时间。
对于长期储存，建议添加载体蛋白。
避免多次冻融循环。

Greater than 90% as determined by -PAGE.

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is <0.3 ng/ml.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.