ProSpec-TNFR Mouse/CYT-774,Tumor Necrosis Factor Receptor Mouse Recombinant

Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tnfrsf1a, Tnfr-1, Tnfr1, FPF, TNF-R, TNFAR, TNFRI, p55-R, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1, TNFalpha-R1.

TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha.
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Human TNFR1 has a major region which controls cell surface expression. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women.
Germline mutations of the extracellular domains of TNFR1 are linked to the autosomal dominant periodic fever syndrome. The impaired receptor clearance is believed to be a mechanism of the disease. Familial hibernian fever is caused by defects in TNFRSF1A gene.

TNFR1 属于 TNF 受体超家族。 TNFR1 是 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。
有 2 种类型的可溶性 TNF 受体：sTNFR-I 和 sTNFR-II，它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
TNFR1 能够发出细胞存活和细胞凋亡的信号。 TNFR1 诱导的细胞凋亡需要 2 个连续的信号复合物。 TNFR1 能够激活 NF-kappaB，介导细胞凋亡，并起到炎症调节剂的作用。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNFR1 有助于诱导非杀细胞性 TNF 效应，包括抗病毒状态和酸性鞘磷脂酶的激活。人 TNFR1 具有控制细胞表面表达的主要区域。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。
TNFR1 细胞外结构域的种系突变与常染色体显性周期性发热综合征有关。受损的受体清除被认为是该疾病的一种机制。家族性冬眠热是由 TNFRSF1A 基因缺陷引起的。

TNFR Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.1kDa.
The TNFR is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFR Mouse Recombinant 是一条单一的、非糖基化的多肽链，含有 191 个氨基酸，分子量为 21.1kDa。
TNFR 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

TNFR protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

It is recommended to reconstitute the lyophilized TNFR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18M-cm H2O 中复溶冻干的 TNFR，然后可以进一步稀释成其他水溶液。

Lyophilized TNFR although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFR 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。 重组后，TNFR 应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 97.0% as determined by -PAGE.

IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A.

The ED₅₀ as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-a.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.