ProSpec-TNFR2 Human Fc/CYT-422,Tumor Necrosis Factor Receptor 2 Fusion Protein Human Recombinant

Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

TNFR binds specifically to tumor necrosis factor and blocks its interaction with cell surface TNF receptors. TNF is a naturally occurring cytokine that is involved in normal inflammatory and immune responses. It plays an important role in the inflammatory processes of rheumatoid arthritis , polyarticular-course juvenile rheumatoid arthritis , and ankylosing spondylitis and the resulting joint pathology. In addition, TNF plays a role in the inflammatory process of plaque psoriasis. Elevated levels of TNF are found in involved tissues and fluids of patients with RA, psoriatic arthritis, ankylosing spondylitis , and plaque psoriasis. Two distinct receptors for TNF , a 55 kilodalton protein and a 75 kilodalton protein , exist naturally as monomeric molecules on cell surfaces and in soluble forms. Biological activity of TNF is dependent upon binding to either cell surface TNFR. Recombinant Human TNFR is a dimeric soluble form of the p75 TNF receptor that can bind to two TNF molecules.
It inhibits the activity of TNF in vitro and has been shown to affect several animal models of inflammation, including murine collagen-induced arthritis. TNFR inhibits binding of both TNF? and TNF? to cell surface TNFRs, rendering TNF biologically inactive. Cells expressing transmembrane TNF that bind to TNFR are not lysed in vitro in the presence or absence of complement.
TNFR can also modulate biological responses that are induced or regulated by TNF, including expression of adhesion molecules responsible for leukocyte migration , serum levels of cytokines , and serum levels of matrix metalloproteinase-3 .

TNFR 特异性结合肿瘤坏死因子并阻断其与细胞表面 TNF 受体的相互作用。 TNF 是一种天然存在的细胞因子，参与正常的炎症和免疫反应。它在类风湿性关节炎、多关节病程幼年类风湿性关节炎和强直性脊柱炎的炎症过程和由此产生的关节病理中起重要作用。此外，TNF 在斑块型银屑病的炎症过程中发挥作用。在 RA、银屑病关节炎、强直性脊柱炎和斑块型银屑病患者的受累组织和体液中发现 TNF 水平升高。两种不同的 TNF 受体，一种 55 千道尔顿的蛋白质和一种 75 千道尔顿的蛋白质，以单体分子的形式自然存在于细胞表面并以可溶形式存在。 TNF 的生物学活性取决于与任一细胞表面 TNFR 的结合。重组人 TNFR 是 p75 TNF 受体的二聚体可溶形式，可与两个 TNF 分子结合。
它在体外抑制 TNF 的活性，并已显示影响多种炎症动物模型，包括小鼠胶原蛋白诱导的关节炎。 TNFR 抑制两种 TNF 的结合？和肿瘤坏死因子？到细胞表面的 TNFR，使 TNF 失去生物活性。在存在或不存在补体的情况下，表达与 TNFR 结合的跨膜 TNF 的细胞不会在体外裂解。
TNFR 还可以调节由 TNF 诱导或调节的生物反应，包括负责白细胞迁移的粘附分子的表达、细胞因子的血清水平和基质金属蛋白酶-3 的血清水平。

Recombinant Human Tumor Necrosis Factor Receptor 2 Fusion Protein produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilo Dalton tumor necrosis factor receptor 2 linked to the Fc portion of human IgG1. The Fc component of TNFR2 contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilo Daltons.
The TNFR2 is purified by standard chromatographic techniques.

在 CHO 中产生的重组人肿瘤坏死因子受体 2 融合蛋白是一种二聚体、糖基化的多肽链，由与人 IgG1 的 Fc 部分连接的人 75 千道尔顿肿瘤坏死因子受体 2 的细胞外配体结合部分组成。 TNFR2 的 Fc 成分包含 CH2 结构域、CH3 结构域和铰链区，但不包含 IgG1 的 CH1 结构域。 它由 934 个氨基酸组成，表观分子量约为 150 千道尔顿。
TNFR2通过标准色谱技术纯化。

Chinese Hamster Ovarian Cells .

Sterile Filtered White lyophilized powder.

Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48 µg tromethamine.

It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFR2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的肿瘤坏死因子受体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TNFR2 应在 4°C 下储存 2-7 天，以备将来在低于 -18°C 的温度下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 98.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17,000,000 IU/mg.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.