TNFR2 Mouse
CYT-770
5µg
20µg
1mg
Catalogue number
CYT-770
Synonyms
Introduction
There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. High levels of soluble TNF receptors are found in the amniotic fluid of pregnant women. TNFR2 and TNFR1 form a heterocomplex which mediates the recruitment of 2 anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. IAPs’ function in TNF-receptor signaling is unknown; nevertheless, c-IAP1 is believed to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury.
有 2 种类型的可溶性 TNF 受体:sTNFR-I 和 sTNFR-II,它们起到中和 TNF α 和 TNF β 的生物活性的作用。这些可溶性受体的水平似乎由于膜结合受体的细胞外结构域脱落而增加。在孕妇的羊水中发现了高水平的可溶性 TNF 受体。 TNFR2 和 TNFR1 形成一个异源复合物,介导 2 种抗凋亡蛋白 c-IAP1 和 c-IAP2 的募集,它们具有 E3 泛素连接酶活性。 IAP 在 TNF 受体信号传导中的功能未知;然而,据信 c-IAP1 通过介导抗凋亡信号的 TNF 受体相关因子 2 的泛素化和降解来增强 TNF 诱导的细胞凋亡。氧化应激促进 TNFR1 和 TNFR2 自我相互作用、配体非依赖性和增强的配体依赖性 TNF 信号传导。 TNF-α、TNFR1和TNFR2在细胞分化中起作用。 TNFR1 和 TNFR2 在细胞类型特异性肾损伤中起作用。
Description
TNFR2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 236 amino acids and having a molecular mass of 25.3kDa.
The TNFR2 is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的 TNFR2 Mouse Recombinant 是单条非糖基化多肽链,含有 236 个氨基酸,分子量为 25.3kDa。
TNFR2 通过专有的色谱技术进行纯化。
Source
Physical Appearance
Formulation
Solubility
Stability
Please prevent freeze-thaw cycles.
请防止冻融循环。
Purity
Amino acid sequence
VPAQVVLTPY KPEPGYECQI SQEYYDRKAQ MCCAKCPPGQ YVKHFCNKTS DTVCADCEAS MYTQVWNQFR TCLSCSSSCT TDQVEIRACT KQQNRVCACE AGRYCALKTH SGSCRQCMRL SKCGPGFGVA SSRAPNGNVL CKACAPGTFS DTTSSTDVCR PHRICSILAI PGNASTDAVC APESPTLSAI PRTLYVSQPE PTRSQPLDQE PGPSQTPSIL TSLGSTPIIE QSTKGG.
Biological Activity
The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 2µg/ml, corresponding to a specific activity of > 500IU/mg in the presence of 0.1ng/mL of rHuTNF-a.
根据其抑制 L-929 细胞中 TNF-a 介导的细胞毒性的能力所确定的 ED50 小于 2μg/ml,对应于在 0.1ng/mL rHuTNF-a 存在下 > 500IU/mg 的比活性 .
Usage
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