ProSpec-TNFRSF10B Human/CYT-069,TNF Ligand Receptor Superfamily Member 10B Human Recombinant

Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, CD262, TNFRSF10B, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, TRICKB, TRICK2A, TRICK2B, KILLER/DR5.

TRAIL Receptor-1 and TRAIL Receptor-2 are members of the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which is capable of activating the cell’s apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L.

TRAIL Receptor-1 和 TRAIL Receptor-2 是跨膜蛋白 TNFR 超家族的成员，含有细胞质“死亡结构域”，能够激活细胞的凋亡机制。 这些受体通过与膜锚定或可溶性 TRAIL/Apo2L 结合而被激活。

TNFRSF10B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 132 amino acids and having a molecular mass of 14.8kDa.
The TNFRSF10B is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TNFRSF10B 人重组体是一种单一的、非糖基化的多肽链，含有 132 个氨基酸，分子量为 14.8kDa。
TNFRSF10B 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH7.4.

It is recommended to reconstitute the lyophilized TNFRSF10B in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议将冻干的 TNFRSF10B 溶解在不低于 100µg/ml 的无菌 18M-cm H2O 中，然后可以进一步稀释成其他水溶液。

Lyophilized TNFRSF10B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFRSF10B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 TNFRSF10B 虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TNFRSF10B 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。 对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by -PAGE.

ESALITQQD LAPQQRVAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KES.

The TNFRSF10B reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS but in the presence of TNFRSF10B, TRAIL-induced TNF is suppressed.

TNFRSF10B 通过中和新鲜人 PBMC 中内源性 TRAIL 的能力来减少 LPS 诱导的 TNF 的产生。 在该试验中，内源性 TRAIL 在 24 小时暴露于 LPS 期间被诱导，但在 TNFRSF10B 存在下，TRAIL 诱导的 TNF 被抑制。

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.