ProSpec-TRAIL Human/CYT-443

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.
In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。 它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。 TRAIL 与死亡受体 DR4 和 DR5 结合。 细胞凋亡过程是 caspase-8 依赖性的。 这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

TRAIL/APO 2 Ligand Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of ~21kDa. The sTRAIL is purified by proprietary chromatographic techniques.

在大肠杆菌中产生的 TRAIL/APO 2 Ligand Human Recombinant 是一种单一的、非糖基化的多肽链，含有 168 个氨基酸，分子量约为 21kDa。 sTRAIL 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a filtered solution containing 20mM Tris-HCl pH 8.0 and 150mM NaCl.

It is recommended to reconstitute the lyophilized TRAIL in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的 TRAIL，然后可以进一步稀释成其他水溶液。

Lyophilized APO 2 Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TRAIL should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.

冻干的 APO 2 配体虽然在室温下可稳定保存 3 周，但应在 -18°C 以下干燥储存。 重组后，TRAIL 应在 4°C 下储存 2-7 天，以备将来在 -18°C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Greater than 95.0% as determined by:
Analysis by RP-HPLC.
Analysis by -PAGE.

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG.

The activity is determined by the cytolysis of Murine L929 cells in the presence of Actinomycin D, ED₅₀ for this effect is less than 2ng/ml, corresponding to a specific activity of 5,000,000IU/mg.

Prospec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.