ProSpec-TRAIL Mouse/CYT-806

TRAIL Mouse

TNF-Related Apoptosis Inducing Ligand/Apo2L Mouse Recombinant
CYT-806
ProSpec-TRAIL Mouse/CYT-806 10µg
ProSpec-TRAIL Mouse/CYT-806 50µg
ProSpec-TRAIL Mouse/CYT-806 1mg

Catalogue number

CYT-806

Synonyms

Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, Apo-2 ligand, Apo-2L, CD253 antigen, TL2, APO2L, TNFSF10.

Introduction

TNF-related apoptosis-inducing ligand is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.

In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.

TNF相关的凋亡诱导配体是诱导细胞凋亡的配体分子。它是一种 II 型跨膜蛋白，与肿瘤坏死因子家族的其他成员具有同源性。
在人类中，编码 TRAIL 的基因位于染色体 3q26。 TRAIL 与死亡受体 DR4 和 DR5 结合。细胞凋亡过程是 caspase-8 依赖性的。这种蛋白质优先诱导转化细胞和肿瘤细胞凋亡，但似乎不会杀死正常细胞，尽管它在大多数正常组织中以显着水平表达。

Description

TRAIL Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 20.2kDa.

在大肠杆菌中产生的 TRAIL 重组小鼠是一条单一的、非糖基化的多肽链，含有 175 个氨基酸，分子量为 20.2kDa。

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized powder.

Formulation

The protein was lyophilized containing PBS, pH 7.4, and 3mM DTT.

Solubility

It is recommended to reconstitute the lyophilized TRAIL Mouse Recombinant in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100 µg/ml 的无菌 18MΩ-cm H2O 中重新配制冻干的 TRAIL 小鼠重组体，然后可以进一步稀释成其他水溶液。

Stability

Lyophilized TRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TRAIL recombinant should be stored at 4C between 2-7 days and for future use below -18C.

For long term storage it is recommended to add a carrier protein .

Please prevent freeze-thaw cycles.

冻干的 TRAIL 虽然在室温下可稳定保存 3 周，但应在 -18C 以下干燥储存。重组后，TRAIL 重组体应在 4C 下储存 2-7 天，以备将来在 -18C 以下使用。
对于长期储存，建议添加载体蛋白。
请防止冻融循环。

Purity

Greater than 95.0% as determined by:

Analysis by RP-HPLC.

Analysis by -PAGE.

Amino acid sequence

MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg in the presence of actinomycin D.

与标准相比具有完全的生物活性。使用鼠 L929 细胞通过细胞毒性试验确定的 ED50 小于 0.5 ng/ml，对应于在放线菌素 D 存在下 > 2,000,000 IU/mg 的比活性。

Usage

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.