ProSpec-Visfatin Human, His/CYT-563

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus . Adipose tissue produces several proteins such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established.
Visfatin, also known as pre-B cell colony-enhancing factor , is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.
Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

过度肥胖是发生胰岛素抵抗和 2 型糖尿病的最重要风险。脂肪组织产生几种蛋白质，如瘦素、脂联素、抵抗素、肿瘤坏死因子-α和 IL-6，它们调节胰岛素敏感性，似乎在胰岛素抵抗、糖尿病、血脂异常、炎症和动脉粥样硬化的发病机制中起重要作用.然而，脂肪组织诱导胰岛素抵抗的机制以及脂肪细胞因子在 T2DM 发病机制中的作用尚未完全确定。
Visfatin，又称pre-B细胞集落增强因子，是一种在内脏脂肪中高度表达的细胞因子，最初作为一种分泌因子被分离出来，与IL-7和干细胞因子协同促进B细胞前体的生长.已在鲤鱼、无脊椎动物软体动物和细菌以及脊椎动物（包括人类和小鼠）中鉴定出 Visfatin 同源物。它被假定在先天免疫中发挥作用。
Visfatin 在刺激肌肉和脂肪细胞葡萄糖转运以及抑制肝细胞葡萄糖产生方面发挥剂量依赖性和数量上与胰岛素相似的胰岛素模拟作用。在小鼠中静脉注射重组内脂素以剂量依赖性方式降低血浆葡萄糖。与其类似胰岛素的作用相一致，visfatin 在降低胰岛素缺陷型糖尿病小鼠的高血糖方面与胰岛素一样有效。还发现 Visfatin 与胰岛素受体结合并激活胰岛素受体，导致受体磷酸化和下游信号分子的激活。然而，内脂素和胰岛素没有竞争与胰岛素受体的结合，表明这两种蛋白质被受体的不同区域识别。因此，内脂素可能在葡萄糖稳态和生物合成或信号转导中的失调中发挥作用，并可能有助于糖尿病的发病机制。

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 511 amino acids and having a molecular mass of 57 kDa.
The recombinant human Visfatin is fused to His tag at N-Terminus.

在大肠杆菌中产生的 Visfatin Human Recombinant 是一种单一的、非糖基化的多肽链，含有 511 个氨基酸，分子量为 57 kDa。
重组人 Visfatin 在 N 末端与 His 标签融合。

Escherichia Coli.

Sterile Filtered solution at a concentration of 1mg/ml.

Visfatin His tag protein contains 20mM Tris pH-8, 0.1mM DTT & 10% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

如果整个小瓶将在 2-4 周内使用，请在 4°C 下储存。
储存，在 -20°C 下冷冻更长时间。
请避免冻融循环。

Greater than 95.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVV YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。