ProSpec-EMAP II Human/CYT-607

AIMP1, EMAP2, EMAP-2, EMAPII, SCYE1, Multisynthetase complex auxiliary component p43, Endothelial monocyte-activating polypeptide 2, EMAP-II, p43.

EMAP-II also called SCYE1 is a tumor derived cytokine that plays a role in a wide variety of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. EMAP-II clinical value is inhibiting angiogenesis of vascular beds and suppressing the growth of primary and secondary tumors with no affect to normal tissues. SCYE1is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this SCYE1 renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. EMAP-2 plays a role in in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells.

EMAP-II 也称为 SCYE1 是一种源自肿瘤的细胞因子，它在内皮细胞、单核细胞和中性粒细胞的多种活动中发挥作用。 EMAP-II 抑制内皮细胞增殖、血管生成、新血管形成，并可诱导细胞凋亡。它还对嗜中性粒细胞和单核细胞具有趋化性，并诱导嗜中性粒细胞的髓过氧化物酶活性。 EMAP-II的临床价值是抑制血管床的血管生成，抑制原发性和继发性肿瘤的生长，对正常组织无影响。 SCYE1 由细胞凋亡特异性诱导，参与控制血管生成、炎症和伤口愈合。这种 SCYE1 的释放使肿瘤相关脉管系统对肿瘤坏死因子敏感​​。前体蛋白与 p43 亚基相同，与多 tRNA 合成酶复合物相关，它调节正常细胞中 tRNA 合成酶的氨酰化活性。 EMAP-2 在肿瘤细胞蛋白水解切割后刺激炎症反应中起作用。

EMAP-II Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 18.3 kDa.
The EMAP-II is purified by proprietary chromatographic techniques.

EMAP-II Recombinant Human 在大肠杆菌中产生，是一条单一的、非糖基化的多肽链，含有 166 个氨基酸，分子量为 18.3 kDa。
EMAP-II 通过专有的色谱技术进行纯化。

Escherichia Coli.

Sterile Filtered White lyophilized powder.

Lyophilized from a concentrated solution in water containing 20mM sodium Phosphate buffer pH=7.5 and 130mM sodium chloride.

It is recommended to reconstitute the lyophilized EMAP-II in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

建议在不低于 100µg/ml 的无菌 18MΩ-cm H2O 中复溶冻干的 EMAP-II，然后可以进一步稀释成其他水溶液。

Lyophilized EMAP-II although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EMAP-II should be stored at 4°C between 2-7 days and for future use below
-18°C.
Please prevent freeze-thaw cycles.

Greater than 98.0% as determined by Analysis by RP-HPLC.
Analysis by -PAGE.

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQM QNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.

Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-30 ng/ml.

ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

ProSpec 的产品仅供实验室研究使用。 该产品不得用作药物、农业或农药产品、食品添加剂或家用化学品。