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- dsGreen Gel Staining Solution, 10000×
dsGreen Gel Staining Solution, 10000×
标签：PCR and qPCR,DNA
| 货号 | 规格 | 价格 | 货期 |
|---|---|---|---|
| A0010 | 50 uL | 29.00$ | in stock |
| 10010 | 0.5 mL | 100.00$ | in stock |
| 20010 | 1 mL | 180.00$ | in stock |
dsGreen is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.
Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.
dsGreen 是一种灵敏的 dsDNA 结合染料，可用于琼脂糖和聚丙烯酰胺凝胶中的常规 DNA 检测。 还提供 qPCR 级试剂。
与溴化乙锭不同，dsGreen 对双链 DNA 具有高度选择性，危害更小，灵敏度更高。
Comparison between ethidium bromide and dsGreen
溴化乙锭和dsGreen的比较
| Feature | Ethidium Bromide | dsGreen |
|---|---|---|
| Fluorescence | Red (615 nm) | Green (524 nm) |
| Excitation maximum | 302 nm | 454 nm |
| Excitation light source | UV only | Blue light or UV |
| Sensitivity | 2 ng / band (dsDNA) 100 ng / band (RNA) |
0.08 ng / band (dsDNA) 1–2 ng / band (oligonucleotides) |
| Health hazard | High | Low |
Excitation and emission spectra of dsDNA complex with dsGreen
dsGreen Gel Staining Solution, 10000×相关产品
Cyanine7 carboxylic acid
Non-reactive, near-infrared fluorescent dye.
非反应性近红外荧光染料。
sulfo-Cyanine3 DBCO
Sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water soluble, general purpose fluorophore sulfo-Cyanine3 for Click Chemistry.
Sulfo-Cy3 DBCO 是用于 Click Chemistry 的水溶性通用荧光团 sulfo-Cy3 的环炔衍生物。
Sulfo-Cyanine5 carboxylic acid
Water-soluble Cyanine5 carboxylic acid, non-activated fluorescent dye.
水溶性Cy5羧酸，非活化荧光染料。
General properties
| Appearance: | orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
储存：收到后 24 个月，在 -20°C 避光保存。 运输：在室温下长达 3 周。 避免长时间暴露在光线下。 |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 454 |
| ε, L⋅mol−1⋅cm−1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0.8 |
相关文献：
- Barrios-Hernández, M.L.; Bettinelli, C.; Mora-Cabrera, K.; Vanegas-Camero, M.-C.; Garcia, H.; van de Vossenberg, J.; Prats, D.; Brdjanovic, D.; van Loosdrecht, M.C.M.; Hooijmans, C.M. Unravelling the removal mechanisms of bacterial and viral surrogates in aerobic granular sludge systems. Water Research, 2021, 195, 116992. doi: 10.1016/j.watres.2021.116992
- Chetverikov, P.E.; Craemer, C.; Cvrković, T.; Klimov, P.B.; Petanović, R.U.; Romanovich, A.E.; Sukhareva, S.I.; Zukoff, S.N.; Bolton, S.; Amrine, J. Molecular phylogeny of the phytoparasitic mite family Phytoptidae (Acariformes: Eriophyoidea) identified the female genitalic anatomy as a major macroevolutionary factor and revealed multiple origins of gall induction. Experimental & Applied Acarology, 2021, 83(1), 31–68. doi: 10.1007/s10493-020-00571-6
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- Kirkham, C.M.; Scott, J.N.F.; Wang, X.; Smith, A.L.; Kupinski, A.P.; Ford, A.M.; Westhead, D.R.; Stockley, P.G.; Tuma, R.; Boyes, J. Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome Instability. Molecular Cell, 2019, 74(3), 584–597.e9. doi: 10.1016/j.molcel.2019.02.025