- Description
Catalogue number
CYT-222
Synonyms
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, CSF2, GMCSF
Introduction
GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Description
GM-CSF Mouse is purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
Solubility
Stability
For long term storage it is recommended to add a carrier protein .
Please prevent freeze-thaw cycles.
Purity
Analysis by -PAGE.
Amino acid sequence
Biological Activity
Protein content
1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% solution. This value is calculated by the PC GENE computer analysis program of protein sequences .
2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
References
Publication: Mol Ther. 2005 Dec;12:1197-205. Epub 2005 Aug 22. PMID: 16112909
Link: http://www.nature.com/mt/journal/v12/n6/full/mt20051405a.html
Applications: The Mouse GM-CSF used for 2 purposes:
1. As an adjuvant for DNA vaccine which included seven plasmids encoding nine HIV-1 proteins. The mice were injected with the DNA vaccine together with recombinant mouse GM-CSF. 2. Used in Elisa.
2.Title: Neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin.
Publication: Exp Parasitol. 2010 Aug;125:357-62. Epub 2010 Mar 6. PMID: 20211619
Link: http://www.sciencedirect.com/science/article/pii/S001448941000086X
Applications: Used for immunoblotting
3.Title: Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the Aspergillus Burden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis.
Publication: Antimicrob Agents Chemother. 2008 February; 52: 716–718.
Published online 2007 November 5
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224773/?tool=pmcentrez
Applications: GM-CSF as tested a therapeutic potential in a murine model of pulmonary aspergillosis. In summary, this pilot study indicates that GM-CSF administered intranasally may be a novel therapeutic approach for the prevention or treatment of pulmonary fungal infections and may augment the efficacies of antifungal agents.
GM-CSF was given intranasal.
4.Title: Dendritic Cell-Based Therapeutic Vaccination against Myeloma: Vaccine Formulation Determines Efficacy against Light Chain Myeloma
Publication: The Journal of Immunology February 1, 2009 vol. 182 no. 3 1667-1673
Link:
http://www.jimmunol.org/content/182/3/1667.full
5.Title:Pre-clinical Evaluation of a CEA DNA Prime/protein Boost Vaccination Strategy Against Colorectal Cancer.
Publication:Article first published online: 21 JUN 2007 DOI:10.1111/j.1365-3083.2007.01945.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2007.01945.x/full
6.Title:Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the Aspergillus Burden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis.
Publication: First published November 2007, doi: 10.1128/?AAC.00760-07 Antimicrob. Agents Chemother. February 2008 vol. 52 no. 2 716-718
Link:http://aac.asm.org/content/52/2/716.full
Publications: Clinical & Experimental Immunology 143.1 : 139-149.
Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2005.02965.x/epdf
Publications: Microbiology and immunology 51.11 : 1121-1133.
Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.2007.tb03998.x/epdf
Publication: Journal of Pharmacology and Experimental Therapeutics 333.3 : 954-960.
Link: http://jpet.aspetjournals.org/content/333/3/954.full
Usage
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