TET phosphoramidite, 6-isomer

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TET phosphoramidite, 6-isomer

标签:Oligonucleotide synthesis,Phosphoramidite,TET
货号 规格 价格 货期
C8160 100 mg 10 days
D8160 250 mg 340.00$ in stock
F8160 1 g 990.00$ in stock
G8160 5 g 3900.00$ 10 days
H8160 10 g please inquire 10 days

TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.

TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).

TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).

5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via RCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM® 310 Genetic Analyzer.

TET 亚磷酰胺用于合成荧光标记的寡核苷酸,纯 6 异构体。

TET(四氯荧光素)是一种绿色荧光荧光素衍生物(最大吸收波长为 519 nm,最大发射波长为 535 nm)。

TET 亚磷酰胺用于合成荧光标记的引物和用于 qPCR 的杂交探针。 TET 可与 DusQ1 荧光猝灭剂一起使用(可与 500 Å DusQ1 CPG 500 一起使用)。

5′ 标记的引物与未标记的反向引物一起使用,通过 RCR 进行微卫星扩增,然后进行片段分析。 TET 标记的扩增产物可以使用各种毛细管电泳测序仪进行分析,包括 ABI PRISM® 310 遗传分析仪。

Recommendations for using the reagent:


Condensation: 3 min.

Deprotection: standard conditions with 25% aqueous ammonium solution; deprotection time depends on nucleic base profile and their protective properties (deprotection for 17 hours at 55°C removes all protection groups from standard nucleic acids). AMA (1:1 mixture of concentrated aqueous ammonium/40% aqueous methylamine) can be used with ~5% non-fluorescent by-product forming. To avoid formation of the by-product, start deprotection with ammonium (30 min at room temperature), then add an equivalent volume of 40% aqueous methylamine solution and continue deprotection as usual with AMA (10 min at 65°C).

冷凝:3 分钟。

脱保护:标准条件用25%铵水溶液; 脱保护时间取决于核酸碱基谱及其保护特性(在 55°C 脱保护 17 小时会去除标准核酸中的所有保护基团)。 AMA(浓铵水溶液/40% 甲胺水溶液的 1:1 混合物)可用于形成约 5% 的非荧光副产物。 为避免形成副产物,用铵开始脱保护(室温下 30 分钟),然后加入等量体积的 40% 甲胺水溶液,并像往常一样用 AMA 继续脱保护(65°C 下 10 分钟)。

TET phosphoramidite, 6-isomer TET phosphoramidite, 6-isomer TET phosphoramidite, 6-isomer

Absorption and emission spectra of TET

TET phosphoramidite, 6-isomer

TET phosphoramidite, 6-isomer相关产品

JOE phosphoramidite, 6-isomer

JOE dye phosphoramidite for synthesis of labeled oligonucleotides. JOE absorption and emission maxima are between maxima of FAM and TAMRA/ROX dyes.

JOE 染料亚磷酰胺用于合成标记的寡核苷酸。 JOE 吸收和发射最大值介于 FAM 和 TAMRA/ROX 染料的最大值之间。

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

General properties

Appearance: white solid foam
Molecular weight: 981.72
CAS number: 877049-90-6
Molecular formula: C46H54N3Cl4O10P
IUPAC name: 2′,4,7,7′-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl bis(2,2-dimethylpropanoate)
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 519
ε, L⋅mol−1⋅cm−1: 100000
Emission maximum, nm: 535
Fluorescence quantum yield: 0.47
CF260: 0.17
CF280: 0.09

Oligo synthesis details寡核苷酸合成细节



anhydrous acetonitrile (prepare a 0.1 М solution, storage 1 week).

无水乙腈(制备 0.1 М 溶液,储存 1 周)。