R6G phosphoramidite, 6-isomer

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R6G phosphoramidite, 6-isomer

标签:Oligonucleotide synthesis,Phosphoramidite,R6G
货号 规格 价格 货期
C5260 100 mg in stock
D5260 250 mg 190.00$ in stock
F5260 1 g 590.00$ in stock
G5260 5 g 2390.00$ in stock
H5260 10 g please inquire 10 days

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

用于寡核苷酸合成的 R6G 亚磷酰胺,纯 6 异构体。

R6G(罗丹明 6G)是罗丹明家族的一种呫吨染料,具有高荧光量子产率和高摩尔消光系数。 相对于荧光素,6-R6G 的最大吸收 (518 nm) 和发射 (542 nm) 转移到长波区域。

R6G 可用作 DNA 测序和短串联重复 (STR) 分析中的 FRET 受体。 该染料通常用于生产用于定量 PCR 的 TaqMan 探针。 与 HEX 和 JOE 染料相比,R6G 与 BHQ1 猝灭剂组合在分子信标探针中具有更强的猝灭作用,显着降低了 PCR 中的背景荧光。


Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

耦合:10 分钟。

脱保护:叔丁胺水溶液 (1:3 v/v) 在 55°С 下过夜。

由于 R6G 完全且不可逆地降解,因此不得使用氨水和 AMA(30% 氢氧化铵/40% 甲胺水溶液 1:1 v/v)将修饰的寡核苷酸从固相载体上脱保护。

R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer R6G phosphoramidite, 6-isomer

Absorption and emission spectra of R6G, 6-isomer

R6G phosphoramidite, 6-isomer

R6G phosphoramidite, 6-isomer相关产品

ROX azide, 5- isomer

ROX (aka rhodamine X or rhodamine 101) azide for biomolecule labeling through Click Chemistry.

ROX(又名罗丹明 X 或罗丹明 101)叠氮化物,用于通过 Click Chemistry 进行生物分子标记。

Cyanine3 azide

Azide derivative of Cyanine3 fluorescent dye for Click Chemistry.

用于 Click Chemistry 的 Cyanine3 荧光染料的叠氮化物衍生物。

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

General properties

Appearance: Yellowish foam
Molecular weight: 949.93
CAS number: 1355330-47-0
Molecular formula: C46H54F6N5O8P
Solubility: good in DCM, acetonitrile
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 518
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 542
Fluorescence quantum yield: 0.95
CF260: 0.18
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile