Pyrene phosphoramidite dU

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Pyrene phosphoramidite dU

Oligonucleotide synthesis,Phosphoramidite,Pyrene
货号 规格 价格 货期
11460 100 mg 150.00$ in stock
21460 250 mg 320.00$ in stock
41460 1 g 880.00$ in stock
61460 5 g please inquire in stock
Pyrene is a polycyclic aromatic hydrocarbon that is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on the fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about the site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows attaching pyrene fragment to 5′, internal, or 3′ position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

Pyrene 是一种多环芳烃,以其嵌入 DNA 的能力而闻名。 芘具有强烈的蓝色荧光。 其参数很大程度上取决于荧光团微环境。 因此,芘的荧光光谱用于提取芘周围位点的结构信息。 两个非常接近的芘通常形成很容易被准分子荧光检测到的准分子。 芘也可以是其他荧光团(如苝)的 FRET 供体。

使用这种亚磷酰胺,可以通过自动合成将芘引入 DNA。 这种芘亚磷酰胺包含刚性连接到脱氧尿苷的烃部分。 该试剂允许将芘片段连接到 5’、内部或 3′ 位置(使用通用支架)。

这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。

Pyrene phosphoramidite dU Pyrene phosphoramidite dU Pyrene phosphoramidite dU Pyrene phosphoramidite dU

Absorption and emission spectra of pyrene fluorophore

Pyrene phosphoramidite dU

Pyrene phosphoramidite dU相关产品

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.

芘 NHS 酯,用于用芘残基标记生物分子。 芘是一种形成准分子的荧光邻近探针,也是石墨固定的锚定基团。

Sulfo-Cyanine5 maleimide

Thiol reactive, water soluble cyanine dye for Cy5® channel.

用于 Cy5® 的硫醇活性水溶性花青染料 渠道。

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.

胺反应性 Cy5 活化酯,用于标记蛋白质、肽和其他分子。

General properties

Appearance: yellowish foam
Molecular weight: 955.04
CAS number: 199920-17-7
Molecular formula: C57H55N4O8P
Solubility: good in dichloromethane and acetonitrile


Quality control: NMR 1H (95%), 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 260; 282; 365; 392
ε, L⋅mol−1⋅cm−1: 12600; 21900; 16000; 14200
Emission maximum, nm: 460
Fluorescence quantum yield: ~0.1

Oligo synthesis details

Diluent: acetonitrile