Perylene dU phosphoramidite

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Perylene dU phosphoramidite

标签:Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
14460 100 mg 150.00$ in stock
24460 250 mg 320.00$ in stock
44460 1 g 880.00$ in stock
64460 5 g please inquire 10 days

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.

With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis. Perylene is attached to the 5′ position of deoxyuridine (dU) through a triple bond and the fluorophore is electronically coupled to the deoxyuridine base. This coupling of dU and perylene makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent is acetonitrile.

Perylene 是一种明亮且极耐光的荧光多环芳烃 (PAH) 标记,其量子产率接近定量。 由于荧光的低寿命,该探针不会形成准分子。

使用这种亚磷酰胺,可以通过自动寡核苷酸合成将苝引入 DNA。 苝通过三键连接到脱氧尿苷 (dU) 的 5′ 位置,并且荧光团与脱氧尿苷碱基电子耦合。 dU 和苝的这种偶联使荧光对分子的 dU 部分的碱基配对敏感,从而可以区分完美和一个碱基错配的靶标。

这种亚酰胺不需要特殊的处理、偶联或脱保护条件。 推荐的稀释剂是乙腈。

Perylene dU phosphoramidite相关产品

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

Hexaethylene glycol phosphoramidite

Spacer phosphoramidite 18 (hexaethylene glycol amidite) provides a long hydrophilic linker for oligo synthesis.

间隔亚磷酰胺 18(六甘醇酰胺)为寡核苷酸合成提供了一个长的亲水接头。

Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.

用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。

General properties

Appearance: orange solid
Molecular weight: 1005.1
CAS number: 908117-78-2
Molecular formula: C61H57N4O8P
Solubility: good in dichloromethane and acetonitrile
Quality control: NMR 1H, 31P, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 435; 408; 252
ε, L⋅mol−1⋅cm−1: 36000
Emission maximum, nm: 439; 467
Fluorescence quantum yield: 1.0

Oligo synthesis details

Diluent: acetonitrile