FAM Phosphoramidite, 6-Isomer

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FAM Phosphoramidite, 6-Isomer

标签:FAM (Fluorescein), Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
D5160 250 mg in stock
F5160 1 g 190.00$ in stock
G5160 5 g 740.00$ in stock
H5160 10 g 1290.00$ in stock
K5160 50 g please inquire 21 days

Sandard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

Sandard fluorescein (FAM) 亚磷酰胺,用于 5′-末端寡核苷酸标记,高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。

FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer FAM Phosphoramidite, 6-Isomer

FAM absorbance and emission spectra

FAM Phosphoramidite, 6-Isomer

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Catalyst for bioconjugate synthesis by Click Chemistry.

Click Chemistry 用于生物共轭合成的催化剂。

Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

磷酸盐修饰剂 CPG,用于固相合成含有 3 个磷酸基修饰的长(最多 120 聚体)寡核苷酸。

BDP FL azide

BDP FL is a bright and photostable borondipyrromethene dye for fluorescein channel. This is a dye azide for Click Chemistry.

BDP FL 是一种用于荧光素通道的明亮且光稳定的硼二吡咯亚甲基染料。 这是一种用于 Click Chemistry 的染料叠氮化物。

General properties

Appearance: white foam
Molecular weight: 843.94
CAS number: 204697-37-0
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下最多保存 2 周。 避免长时间暴露在光线下。 干燥。
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

相关文献:

  1. Zhou, Z.; Liu, S.; Zhang, Y.; Yang, X.; Ma, Y.; Guan, Z.; Wu, Y.; Zhang, L.; Yang, Z. Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells. International Journal of Nanomedicine, 2017, 12, 7255–7272. doi: 10.2147/ijn.S136726