FAM Phosphoramidite, 5-Isomer

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FAM Phosphoramidite, 5-Isomer

标签:FAM (Fluorescein),Oligonucleotide synthesis,Phosphoramidite
货号 规格 价格 货期
2639-500mg 500 mg 10 days
2639-1g 1 g 190.00$ in stock
2639-5g 5 g 740.00$ in stock
2639-10g 10 g 1290.00$ in stock
2639-50g 50 g please inquire 21 days

Standard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺,高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。

寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。

FAM Phosphoramidite, 5-Isomer FAM Phosphoramidite, 5-Isomer FAM Phosphoramidite, 5-Isomer

FAM Phosphoramidite, 5-Isomer相关产品

Phosphate CPG 500 solid support

CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.

用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。

HEX phosphoramidite, 6-isomer

HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.

用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。

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General properties

Appearance: off white solid
Molecular weight: 843.94
CAS number: 147566-42-5
Molecular formula: C46H58N3O10P
Solubility: Good solubility in acetonitrile and DCM
Quality control: NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: coupling time 10 min
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine


  1. Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212