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- FAM Phosphoramidite, 5-Isomer
FAM Phosphoramidite, 5-Isomer
标签:FAM (Fluorescein),Oligonucleotide synthesis,Phosphoramidite
货号 | 规格 | 价格 | 货期 |
---|---|---|---|
2639-500mg | 500 mg | – | 10 days |
2639-1g | 1 g | 190.00$ | in stock |
2639-5g | 5 g | 740.00$ | in stock |
2639-10g | 10 g | 1290.00$ | in stock |
2639-50g | 50 g | please inquire | 21 days |
Standard fluorescein (FAM) phosphoramidite for 5′-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
用于 5′-末端寡核苷酸标记的标准荧光素 (FAM) 亚磷酰胺,高异构纯度单一异构体。 这种高度纯化的亚酰胺试剂可确保与各种合成器获得出色的偶联效果。
寡核苷酸制造商可提供样品和特别优惠。 联系我们获取该试剂的批量供应。
FAM Phosphoramidite, 5-Isomer相关产品
Phosphate CPG 500 solid support
CPG solid support for the synthesis of 3′-phosphorylated oligonucleotides.
用于合成 3′-磷酸化寡核苷酸的 CPG 固体支持物。
HEX phosphoramidite, 6-isomer
HEX phosphoramidite for synthesis of 5’-labeled oligonucleotides.
用于合成 5′ 标记寡核苷酸的 HEX 亚磷酰胺。
General properties
Appearance: | off white solid |
Molecular weight: | 843.94 |
CAS number: | 147566-42-5 |
Molecular formula: | C46H58N3O10P |
Solubility: | Good solubility in acetonitrile and DCM |
Quality control: | NMR 1H and 31P, HPLC-MS (95+%), isomeric purity > 97% |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。 |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | coupling time 10 min |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine |
相关文献:
- Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212