Cyanine5.5 phosphoramidite, 5′-terminal

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Cyanine5.5 phosphoramidite, 5′-terminal

标签:Cyanine5.5, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2680-100mg 100 mg 7 days
2680-250mg 250 mg 290.00$ 10 days
2680-1g 1 g 950.00$ in stock
2680-10g 10 g 6750.00$ 21 days
Cyanine5.5 is a fluorophore with the emission in far red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.

This phosphoramidite can be used for the synthesis of 5′-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.

The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.

Cy5.5 是一种荧光团,在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。

与仲碳原子相连的亚磷酰胺官能团结构提供了额外的稳定性,可防止 Arbuzov 重排。 与衍生自伯醇的亚磷酰胺相比,这有助于在寡核苷酸合成仪中在较长的存储时间内保持偶联性能。

Absorption and emission spectra of Cyanine5.5

Cyanine5.5 phosphoramidite, 5'-terminal

Cyanine5.5 phosphoramidite, 5′-terminal相关产品

Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

磷酸盐修饰剂 CPG,用于固相合成含有 3 个磷酸基修饰的长(最多 120 聚体)寡核苷酸。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cy5 的亚磷酰胺衍生物,用于寡核苷酸合成。

FAM CPG 500, 6-isomer

Controlled pore glass for the synthesis of modified oligonucleotides containing 3′-fluorescein (FAM) modification.

用于合成含有 3′-荧光素 (FAM) 修饰的修饰寡核苷酸的可控孔玻璃。

General properties

Appearance: dark colored solid
Molecular weight: 916.61
Molecular formula: C55H71N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (85%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 694
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

Cyanine5.5 phosphoramidite, 5'-terminal