Cyanine5.5 phosphoramidite, 5′-terminal

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Cyanine5.5 phosphoramidite, 5′-terminal

标签:Cyanine5.5, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
2680-100mg 100 mg 7 days
2680-250mg 250 mg 290.00$ 10 days
2680-1g 1 g 950.00$ in stock
2680-10g 10 g 6750.00$ 21 days
Cyanine5.5 is a fluorophore with the emission in far red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.

This phosphoramidite can be used for the synthesis of 5′-labeled oligonucleotides by direct labeling in oligonucleotide synthesizer.

The structure of phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintaining coupling performance over a longer storage time in oligonucleotide synthesizer, compared to phosphoramidites derived of primary alcohols.

Cy5.5 是一种荧光团,在光谱的远红色范围内发射。 这种染料可用于多重 qPCR。 商用六通道 qPCR 仪器通常有一个用于 Cy5.5 的通道。
该亚磷酰胺可用于通过在寡核苷酸合成仪中直接标记来合成5′-标记的寡核苷酸。

与仲碳原子相连的亚磷酰胺官能团结构提供了额外的稳定性,可防止 Arbuzov 重排。 与衍生自伯醇的亚磷酰胺相比,这有助于在寡核苷酸合成仪中在较长的存储时间内保持偶联性能。

Absorption and emission spectra of Cyanine5.5

Cyanine5.5 phosphoramidite, 5'-terminal

Cyanine5.5 phosphoramidite, 5′-terminal相关产品

Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

磷酸盐修饰剂 CPG,用于固相合成含有 3 个磷酸基修饰的长(最多 120 聚体)寡核苷酸。

Cyanine5 phosphoramidite

Phosphoramidite derivative of Cyanine5 for the use in oligonucleotide synthesis.

Cy5 的亚磷酰胺衍生物,用于寡核苷酸合成。

FAM CPG 500, 6-isomer

Controlled pore glass for the synthesis of modified oligonucleotides containing 3′-fluorescein (FAM) modification.

用于合成含有 3′-荧光素 (FAM) 修饰的修饰寡核苷酸的可控孔玻璃。

General properties

Appearance: dark colored solid
Molecular weight: 916.61
Molecular formula: C55H71N5ClO3P
Quality control: NMR 1H, 31P, HPLC-MS (85%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

储存:收到后 12 个月,在 -20°C 避光保存。 运输:在室温下长达 3 周。 避免长时间暴露在光线下。 干燥。

MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 694
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: 6 min coupling time recommended
Deprotection conditions: recommended 48 h at +4°C or ultramild protective groups; 24 h at rt possible

Cyanine5.5 phosphoramidite, 5'-terminal