Alkyne Phosphoramidite, 5′-terminal

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Alkyne Phosphoramidite, 5′-terminal

标签:Alkynes, Oligonucleotide synthesis, Phosphoramidite
货号 规格 价格 货期
22260 250 mg 110.00$ in stock
32260 500 mg 190.00$ in stock
42260 1 g 310.00$ in stock
62260 5 g 1290.00$ in stock
82260 10 g please inquire in stock


Phosphoramidite for the synthesis of oligonucleotides with 5′-terminal alkyne for Click Chemistry.

This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5′-butynyl-CEP, and other 5′-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life

Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5′-terminal DMT group, no 5′-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.

Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).

亚磷酰胺用于合成具有 5′-末端炔烃的寡核苷酸,用于 Click Chemistry。

与 5′-己炔基亚磷酰胺、5′-丁炔基-CEP 和其他 5′-末端炔基亚磷酰胺相比,这种炔基亚磷酰胺具有多个优点。 首先,它是更易于处理和分配的固体化合物。 并且由于其结构,在溶液中也更稳定,保质期更长

该亚磷酰胺的稀释剂是乙腈,建议偶联时间为 5 分钟。 因为这种亚酰胺不含 5′-末端 DMT 基团,所以不需要 5′-去保护。 寡核苷酸应在标准条件下解封,并通过 PAGE 或离子交换 HPLC 纯化。

具有这种修饰的寡核苷酸非常适合用于 Click Chemistry(请参阅我们推荐的方案)。

Alkyne Phosphoramidite, 5′-terminal相关产品

ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

用于胺标记的纯异构体 6-ROX NHS 酯。

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

NHS 酯形式的水溶性磺化远红色磺基-Cy5.5 染料。

Biotin phosphoramidite (hydroxyprolinol)

Biotin phosphoramidite reagent for the synthesis of biotinylated oligos. Contains a dimethyoxytrityl group.

用于合成生物素化寡核苷酸的生物素亚磷酰胺试剂。 含有二甲氧基三苯甲基。

General properties

Appearance: colorless solid
Molecular weight: 409.50
CAS number: 1417539-32-2
Molecular formula: C21H36N3O3P
IUPAC name: trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine
Solubility: good in acetonitrile and dichloromethane


Quality control: NMR 1H (95%) and 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

储存:-20°C 收货后 12 个月。 运输:在室温下长达 3 周。 干燥。

MSDS: Download
Product specifications

Oligo synthesis details

Diluent: acetonitrile 乙腈
Coupling conditions: Standard coupling, identical to normal nucleobases


Cleavage conditions: standard deprotection


Deprotection conditions: No deprotection required. Compatible with standard deprotection reagents.

无需脱保护。 与标准脱保护试剂兼容。


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