• Products
• DNA
• Phosphoramidites
• Alkyne Phosphoramidite, 5′-terminal

Alkyne Phosphoramidite, 5′-terminal

Phosphoramidite for the synthesis of oligonucleotides with 5′-terminal alkyne for Click Chemistry.

This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5′-butynyl-CEP, and other 5′-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life

Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5′-terminal DMT group, no 5′-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.

Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).

亚磷酰胺用于合成具有 5′-末端炔烃的寡核苷酸，用于 Click Chemistry。

与 5′-己炔基亚磷酰胺、5′-丁炔基-CEP 和其他 5′-末端炔基亚磷酰胺相比，这种炔基亚磷酰胺具有多个优点。 首先，它是更易于处理和分配的固体化合物。 并且由于其结构，在溶液中也更稳定，保质期更长

该亚磷酰胺的稀释剂是乙腈，建议偶联时间为 5 分钟。 因为这种亚酰胺不含 5′-末端 DMT 基团，所以不需要 5′-去保护。 寡核苷酸应在标准条件下解封，并通过 PAGE 或离子交换 HPLC 纯化。

具有这种修饰的寡核苷酸非常适合用于 Click Chemistry（请参阅我们推荐的方案）。